Preparation of Reference Material for Quantitative Detection of Escherichia Coli
XUE Lei1,2,LIN Jing3,SUI Zhi-wei1,WANG Jing1,LIU Xiao-xia1,2, FU Bo-qiang1,ZHANG Ling1,HAO Lin2
1. National Institute of Metrology, Beijing 100029, China;
2. College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030800, China;
3. Nanjing Institute of Measurement and Testing Technology, Nanjing, Jiangsu 210049, China
Abstract：The protective effect of freeze-dried protectant, homogeneity and stability of the escherichia coli reference material are verified by plate count method. The results show that the protection of the composite protective agent reach 53.19%, and it can effectively protect the escherichia coli during freeze-drying. After escherichia coli and the freeze-dried protectant mixed, frozen, dried and prepared a dry powder reference materials of escherichia coli,its shape, rehydration speed and homogeneity are good, and it can be stable for six months. The reference material can be used to quality control of food testing laboratory and evaluation of food test results.
［1］GB 19644—2010 食品安全国家标准 乳粉［S］.
［2］倪晓丽. 生物标准物质的研制现状及展望［J］. 中国计量, 1999, (12): 47-48.
［3］GB 47893—2010 食品安全国家标准 食品微生物学检验 大肠菌群计数［S］.
［4］陈雪华. 应用VITEK2全自动微生物鉴定系统检测养殖用水中的大肠杆菌［J］. 热带农业科学, 2012, 32(5):76-80.
［5］田芬, 陈俊亮, 霍贵成. 益生菌冻干保护剂优化及菌粉保存稳定性研究［J］. 食品科技, 2012, 37(2): 15-19.
［6］Tessema D A, Rosen R, Pedazur R, et al. Freeze-drying of sol—gel encapsulated recombinant bioluminescent E. coli by using lyo-protectants［J］. Sensors and Actuators B: Chemical, 2006, 113(2): 768–773.
［7］Zayed G, Roos Y H. Influence of trehalose and moisture content on survival of lactobacillus salivarius subjected to freeze-drying and storage［J］. Process Biochemistry, 2004, 39(9):1081–1086.
［8］陈合, 李串娜, 舒国伟, 等. 正交法优化保加利亚乳杆菌冻干保护剂［J］.陕西科技大学学报, 2013,31 (2): 89-92.
［9］Yang C Y, Zhu X L, Fan D D, et al. Optimizing the chemical compositions of protective agents for freeze drying bifidobacterium longum BIOMA 5920［J］. Chinese Journal of Chemical Engineering, 2012, 20(5): 930-936.
［10］吴谦, 黄晓蓉, 王晶, 等. 鸡肉中单核细胞增生李斯特氏菌标准物质的研制［J］. 计量学报, 2010, 31(5A): 9-13.
［11］鲍行豪, 沈为民, 沈永芳. 微生态制品中双歧杆菌活存因素的探讨［J］. 中国微生态学杂志,1997,9(6):20-23.
［12］姚祖华,李亦德. 生态制品的冻干及保护剂的应用［J］. 中国微生态学杂志, 1997,9(6):48-49.
［13］国家药典委员会.中华人民共和国药典（2010版）［M］. 北京:中国医药科技出版社, 2010.
［14］GB 29921—2013 食品中致病菌限量［S］.
［15］陈彬, 郑晶, 黄晓蓉, 等. 乳粉中阪崎肠杆菌标准物质的研制 ［J］. 中国乳品工业, 2012, 40(12): 16-18.
［16］Wang W. Lyophilization and development of solid protein pharmaceuticals［J］. International Journal of Pharmaceutics, 2000, 203(1-2): 1-60.
［17］Finkel S E. Long-term survival during stationary phase: evolution and the GASP phenotype［J］. Nature Reviews Microbiology, 2006, 4(2): 113-120.
2015, Vol. 36 
