Abstract：Immunochip is designed in order to realize the massive detection of clenbuterol residue.Slide glass surface as the carrier is modified by APTES-DGA.The clenbuterol antibody is coupled to the slide glass surface.Glass surface active groups are blocked by bovine serum albumin. The samples and standards, horseradish peroxidase labeled clenbuterol are added into the immunochip.CLB and CLB-HRP are combined competitively with Ab (CLB).Chemiluminescence reaction is catalyzed by the horseradish peroxidase and optical signal value is detected Sampling volume, incubation temperature, incubation time, concentration of washing solution, chemiluminescence integral time are tested.The results show that glass slide with APTESDGA has a better signal uniformity and antibody coupling ability. In the detection process, immunochip signal is stable when sampling volume is 50 μL, incubation temperature is 37 ℃, incubation time is 30 min, wash solution concentration is 10 fold diluent and chemiluminescence reader integral time is 60 s. The method is fast, simple and accurate by immunochip analysis for clenbuterol residue.
孙来玉,钱坤,邵朝纲,尹莉,严琼琼,陆云华,杨金田. 克伦特罗残留检测免疫芯片的研制[J]. 计量学报, 2015, 36(6): 657-661.
SUN Lai-yu,QIAN Kun,SHAO Chao-gang,YIN Li,YAN Qiong-qiong, LU Yun-hua,YANG Jin-tian. Development of Immunochip for Detection of Clenbuterol Residue. Acta Metrologica Sinica, 2015, 36(6): 657-661.
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2015, Vol. 36 
