Research Progress of Next-Generation Sequencing Reference Materials
LIU Yang1,2,SU Zhaozhong1,2,ZENG Fanjun3,YUAN Huijun1,ZHANG Yongzhuo2
1. College of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu 730050, China
2. National Institute of Metrology, Beijing 100029, China
3. International Travel Health Care Center, General Administration of Customs (Beijing) , Beijing 100013, China
Abstract：With the advent of next-generation sequencing technology, gene sequence has been widely studied.Due to the complexity of genetic material, technical errors introduced during sample preparation, sequencing, and analysis, and systematic biases between different sequencing platforms, high-throughput sequencing results suffer in terms of accuracy and consistency across platforms. However, the use of standard materials in the sequencing process can solve these problems. Next-generation sequencing reference materials are usually genetic materials with good characteristics or synthetic reference materials added and simulated electronic data sets. The application of next-generation sequencing reference materials will help calibrate the measurement results of next-generation sequencing and evaluate the instrument performance, which is crucial to ensure the accuracy and consistency of sequencing results.
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