高通量测序标准物质的研究进展

刘杨; 苏照中; 曾凡俊; 袁惠君; 张永卓

doi:10.3969/j.issn.1000-1158.2024.01.18

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计量学报 ›› 2024, Vol. 45 ›› Issue (1) : 128-134. DOI: 10.3969/j.issn.1000-1158.2024.01.18

电离辐射、化学计量与生物计量

高通量测序标准物质的研究进展

刘杨^1,2,苏照中^1,2,曾凡俊³,袁惠君¹,张永卓²

作者信息 +

Research Progress of Next-Generation Sequencing Reference Materials

LIU Yang^1,2,SU Zhaozhong^1,2,ZENG Fanjun³,YUAN Huijun¹,ZHANG Yongzhuo²

Author information +

文章历史 +

摘要

高通量测序技术的出现使基因序列得到了广泛研究。由于遗传物质的复杂性以及在样品准备、测序和分析过程中引入的技术错误和不同测序平台之间的系统偏差,导致高通量测序结果在准确性和各平台一致性方面受到影响。而在测序流程中使用标准物质可以很好地解决这些问题。高通量测序标准物质通常是具有良好特征的遗传物质或合成的添加对照物和模拟的电子数据集,高通量测序标准物质的应用将有助于校准高通量测序的测量结果和评估仪器性能,对确保测序结果的准确性、一致性至关重要。

Abstract

With the advent of next-generation sequencing technology, gene sequence has been widely studied.Due to the complexity of genetic material, technical errors introduced during sample preparation, sequencing, and analysis, and systematic biases between different sequencing platforms, high-throughput sequencing results suffer in terms of accuracy and consistency across platforms. However, the use of standard materials in the sequencing process can solve these problems. Next-generation sequencing reference materials are usually genetic materials with good characteristics or synthetic reference materials added and simulated electronic data sets. The application of next-generation sequencing reference materials will help calibrate the measurement results of next-generation sequencing and evaluate the instrument performance, which is crucial to ensure the accuracy and consistency of sequencing results.

导出引用

刘杨,苏照中,曾凡俊,袁惠君,张永卓. 高通量测序标准物质的研究进展[J]. 计量学报. 2024, 45(1): 128-134 https://doi.org/10.3969/j.issn.1000-1158.2024.01.18

LIU Yang,SU Zhaozhong,ZENG Fanjun,YUAN Huijun,ZHANG Yongzhuo. Research Progress of Next-Generation Sequencing Reference Materials[J]. Acta Metrologica Sinica. 2024, 45(1): 128-134 https://doi.org/10.3969/j.issn.1000-1158.2024.01.18

中图分类号： TB99

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