Development of Certified Reference Materials for Evaluation on Protection Performance of Biological Safety Cabinet
WANG Zi-quan1,LIU Si-yuan1,ZHEN Xiao-xiao2,HUNAG Wen-feng1,ZHANG Ling1,XU Qian3,4,SUI Zhi-wei1
1. Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China
2. Beijing Institute of Metrology, Beijing 100029, China
3. College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China
4. Hunan Institute of Metrology and Test, Changsha, Hunan 410014, China
Abstract：The protection performance of biosafety cabinet not only affects the accuracy and reliability of experimental results, but also relates to the personal safety of laboratory personnel, therefore its protection performance needs to be evaluated and calibrated in time. Bacillus subtilis (B. subtilis) is an indicator of performance evaluation specified in the domestic and international standards of biosafety cabinet, thus development of B. subtilis reference material is necessary. Three reference materials were prepared by freeze-drying B. subtilis spores in the presence of a composite cryoprotectant. The result indicated that the reference materials of B. subtilis spores count bearing excellent homogeneity and could be stored stably for twenty-four months at 4℃. The certified values of three different concentrations of certified reference materials were (5.4±1.3)×108CFU/mL, (5.2±1.3)×106CFU/mL, (5.3±1.4)×104CFU/mL, respectively. The reference materials of B. subtilis spores count could mainly be used to evaluate the staff, product, and cross-contamination protection of the biosafety cabinet.
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2023, Vol. 44 
