鸡肉中非典型沙门氏菌标准物质的研制及其在能力验证中的应用

doi:10.3969/j.issn.1000-1158.2023.07.20

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摘要针对目前沙门氏菌标准物质种类缺乏、研制水平有待提高的问题,选取非典型沙门氏菌NIM JZ048作为目标菌,产气肠杆菌CICC 10293作为干扰菌,研制鸡肉中非典型沙门氏菌标准物质。结果表明,该标准物质的沙门氏菌定量标准值为(1.8±0.4)×10³CFU/g,菌落总数定量标准值为(5.5±0.9)×10³CFU/g,定性标准值为沙门氏菌阳性。均匀性和稳定性良好,可以在-20℃下稳定保存6个月。此外,将该标准物质作为考核样品用于21家食品承检机构的沙门氏菌定性检测能力验证,其中15家结果合格,其余6家机构因非典型沙门氏菌漏检,结果不合格。制备的鸡肉中非典型沙门氏菌标准物质可以有效用于食品承检机构的沙门氏菌检测能力评价。

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关键词 ：计量学, 沙门氏菌, 标准物质, 能力验证

Abstract：In order to solve the problem of the lack of reference material and the development level of Salmonella, atypical Salmonella NIM JZ048 was selected as the target bacteria, and Enterobacter aerogenes CICC 10293 was selected as the interference bacteria to develop atypical Salmonella reference materials in chicken.The results indicated that the quantitative standard value of Salmonella for the reference material is (1.8±0.4)×10³CFU/g, the quantitative standard value of total bacterial count is (5.5±0.9)×10³CFU/g, the qualitative standard value is Salmonella positive. The reference material has good uniformity and stability, and can be stably stored at -20℃ for six months.In addition, the reference material was used as the test sample for proficiency test of Salmonella qualitative detection participated by twenty-one food inspection agencies. The results of 15 agencies were qualified. Six agencies failed to detect atypical Salmonella, resulting in unqualified results. The atypical Salmonella reference material in chicken can be effectively used for the evaluation of Salmonella detection capability in food inspection agencies.

Key words： metrology Salmonella reference material proficiency testing

收稿日期: 2021-08-03 发布日期: 2023-07-17

PACS:

TB99

基金资助:国家重点研发计划(2017YFF0204602);国家市场监督管理总局科技计划(2019MK114);中国计量科学研究院基本业务费(AKYZD2110, AKY1958)

通讯作者: 隋志伟(1980-),山西太原人,中国计量科学研究院副研究员,博士,主要从事微生物计量研究。Email: suizhiwei_2001@163.com E-mail: suizhw@nim.ac.cn

作者简介: 徐倩(1997-),湖南邵东人,湖南师范大学硕士研究生,主要研究方向为微生物计量方法。Email:1870688307@qq.com

引用本文:

徐倩,刘思渊,高颖,王梓权,隋志伟,常海艳. 鸡肉中非典型沙门氏菌标准物质的研制及其在能力验证中的应用[J]. 计量学报, 2023, 44(7): 1134-1140.
XU Qian,LIU Si-yuan,GAO Ying,WANG Zi-quan,SUI Zhi-wei,CHANG Hai-yan. Preparation of Atypical Salmonella Reference Material in Chicken and the Application in the Proficiency Testing. Acta Metrologica Sinica, 2023, 44(7): 1134-1140.

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http://jlxb.china-csm.org:81/Jwk_jlxb/CN/10.3969/j.issn.1000-1158.2023.07.20 或 http://jlxb.china-csm.org:81/Jwk_jlxb/CN/Y2023/V44/I7/1134

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