Abstract：To construct a plasmid pUC57-BT11 including event specific product and endogenous gene zSSIIb product.After restriction enzyme digestion and sequencing, the specific of the plasmid pUC57-BT11 is detected.The property value of pBT11 is 1.01±0.01(k=2) determined by sequencing and real time PCR.By comparison standard curve generated by plasmid with genomic DNA, no significant difference between the slopes of the standard curve is found.All correlated coefficient R2 are ≥0.99.Certified GM matrix materials are detected by quantitative PCR when pBT11 plasmid DNA is used as quantification standards.The results determined by quantitative PCR with pBT11 are identical with the certified values.This indicated that pBT11 plasmid can be used as a standard for GM maize BT11 quantitative and qualitatively detection.
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2011, Vol. 32 
