Determination of Ampicillin and Amoxicillin Residues in Aquatic Product Using Reversed-phase High Performance Liquid Chromatography-fluorescence Detection with Pre-column Derivatization
WANG Ping-ya1,ZHOU Yong1,HUANG Li1,ZHAO Hua1,XUE Chao-bo1,XU Zhen-jian1,FANG Yi1,CHEN Zhong-wei2
1. National Marine Food Quality Supervision and Testing Center, Zhoushan, Zhejiang 316021, China
2. Zhoushan Agency for Public Health Inspection, Zhoushan, Zhejiang 316021, China
Abstract：A methd is developed for determination of ampicillin and amoxicillin residues in aquatic product using reversed-phase high performance liquid chromatography-fluorescence detection with pre-column derivatization.The sample extracts are cleaned up by solid-phase extraction cartridge and then derivatized with derivative agent.The results show that the calibration graphs is linear in the concentration rang of 0.001~0.1 mg/kg（r＝0.999 9）. The detection limit and quantification limit are 0.001 mg/kg（S/N=3） and 0.003 mg/kg（S/N=10） for ampicillin, 0.005 mg/kg（S/N=3） and 0.015 mg/kg（S/N=10） for amoxicillin.The average recoveries are between 76.3%~96.8% with relative standard deviations（RSDs） of 0.63%~6.71%（n=6）. The method is simple,rapid,sensitive and reproducible,it can be used for the routine analysis of the ampicillin and amoxicillin residues in aquatic product.
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2012, Vol. 33 
