Abstract：In order to calibrate the temperature of PCR instrument, a method was introduced for thermistor encapsulation, and a temperature calibration system for PCR instrument based on multi-thermistor sensors was developed, also the verification experiment for the system was accomplished. The experimental results demonstrated that the uniformity and the absolute value of indication error of the calibration system developed was below 0.15℃ and 0.06℃ respectively. While the extended uncertainty for indication error was less than 0.07℃ (k=2). In addition, a PCR instrument was calibrated using the developed system, and the parameters, such as indication error, uniformity, heat rate and cool rate, were all obtained.
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2018, Vol. 39 
