Development of Quantum Dot-Doped Fluorescent Gel Reference Strip and Its Application in Calibration of Fluorescence Immunoassay Analyzer
TAO Qing1,WANG Le-le1,PENG Tao2,LI Lan-ying1,LIU Gang1,WEN Yan-li1
1. Key Laboratory of Bioanalysis and Metrology for State Market Regulation, Shanghai Institute of Measurement and Testing Technology, Shanghai 201203, China
2. National Institute of Metrology, Beijing 100029, China
Abstract：Quantum dot-doped fluorescent gel reference strips were developed based on microfluidics and quantum dot technology. These strips were formed by uniformly mixed quantum dots and PDMS, which were pressed into capillary tubes to solidify. By controlling the concentration of quantum dots, fluorescent gel reference strips with three fluorescence intensities of high, medium, and low were prepared. The photobleaching, homogeneity, and stability of the reference strips were investigated, and the uncertainties were evaluated. The results showed that the fluorescent gel reference strips possessed good photobleaching resistance and uniformity, and can be stored stably for 180 days at room temperature in the dark. Calibration experiments on a number of fluorescent immunoassay analyzers proved that the fluorescent gel reference strips had good applicability and provided a reliable reference for the calibration of fluorescent immunoassay analyzers.
陶晴,王乐乐,彭涛,李兰英,刘刚,闻艳丽. 量子点掺杂荧光凝胶标准试纸条研制及荧光免疫分析仪校准应用[J]. 计量学报, 2023, 44(12): 1919-1924.
TAO Qing,WANG Le-le,PENG Tao,LI Lan-ying,LIU Gang,WEN Yan-li. Development of Quantum Dot-Doped Fluorescent Gel Reference Strip and Its Application in Calibration of Fluorescence Immunoassay Analyzer. Acta Metrologica Sinica, 2023, 44(12): 1919-1924.
Zhang J R, Qu Y G, Fan X X, et al. Study on the application of the hypersensitive fluorescence immunoassay in detecting antibodies against mycobacterium bovis［J］. Pathogens, 2022, 39 (11): 121-125.
［4］
Hu G, Sheng W, Li S, et al. Quantum dot based multiplex fluorescence quenching immune chromatographic strips for the simultaneous determination of sulfonamide and fluoroquinolone residues in chicken samples ［J］. RSC Advances, 2017, 7 (49): 31123-31128.
［5］
Li X T, Jiang K, Wang P. Research of Quantitative Analyzer for Fluorescence Immune Chromatography Strips ［J］. Applied Mechanics and Materials, 2013, 373: 1000-1006.
［7］
Wang C, Cheng X, Liu L, et al. Ultrasensitive and Simultaneous Detection of Two Specific SARS-CoV-2 Antigens in Human Specimens Using Direct/Enrichment Dual-Mode Fluorescence Lateral Flow Immunoassay ［J］. ACS Applied Materials & Interfaces, 2021, 13 (34): 40342-40353.
Zhang Y, Zhou Y, Zhou Y, et al. Development and application of an indirect fluorescent immunochromatographic assay for quantitative detection of PLA2R-IgG ［J］. Progress in Biochemistry and Biophysics, 2022, 49 (9): 1802-1809.
Yin H, Meng Y, Peng W, et al. Application of rare-earth-elements-doped upconversion nanoparticles in the fields of pesticide residue detection ［J］. Chemical Reagents, 2023, 45 (3): 22-28.
Brunner C, Hoffmann K, Thiele T, et al. Novel calibration tools and validation concepts for microarray-based platforms used in molecular diagnostics and food safety control ［J］. Analytical and Bioanalytical Chemistry, 2015, 407 (11): 3181-3191.
［17］
Arakawa Y, Holmes M J. Progress in quantum-dot single photon sources for quantum information technologies: A broad spectrum overview ［J］. Applied Physics Reviews, 2020, 7 (2): 021309-0213024.
［6］
Li G, Guan F, Zhao S, et al. Immediate, sensitive and specific time-resolved fluorescent immunoassay strips based on immune competition for the detection of procymidone in vegetables ［J］. Food Control, 2023, 147: 109569-109579.
Chen J, Fu W Z, Liang C Y. Safety and effectiveness of dry fluorescence immunoassay analyzer ［J］. Medical Equipment, 2019, 31 (11): 35-36.
Wan Z. Discussion on calibration methods for dry fluorescence immunoassays ［J］. Branding and standardization, 2015, 10: 78-79.
Yang Z, Ling T X, Zhang J. Photo-bleaching effects of different parameters of laser confocal microscopy［J］. Journal of Chinese Electron Microscopy Society, 2021, 40 (6): 704-710.
［15］
Zhou R, Lu X, Yang Q, et al. Nanocrystals for large Stokes shift-based optosensing ［J］. Chinese Chemical Letters, 2019, 30 (10): 1843-1848.
Xu P P, Zhang L, Huang F, et al. Effect of fluorescence properties of fluorescent film materials on the measurement results of fluorescence microscope analysis ［J］. Acta Metrologica Sinica, 2019, 40 (4): 742-748.
Wang G X, Liu J. Performance verification and measurement requirements of the fluorescence immunochromatography analyzer ［J］. Metrology and Testing Technology, 2022, 49 (3): 43-45.
Xie M Y, Zhang J, Gu W, et al. Research on portable quantitative fluorescence immunoassay analyzer ［J］. Instrument Technique and Sensor, 2014, 12: 25-28.
［18］
Garcia de Arquer F P, Talapin D V, Klimov V I, et al. Semiconductor quantum dots: Technological progress and future challenges ［J］. Science, 2021, 373 (6555): 1-14.
2023, Vol. 44 
