基于荧光法生物气溶胶监测仪计数效率评价及方法研究

doi:10.3969/j.issn.1000-1158.2023.02.18

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摘要生物气溶胶监测仪是一类快速、实时监测空气中生物气溶胶的新兴仪器。为系统评价生物气溶胶监测仪的计数效率,开展了荧光法生物气溶胶监测仪总粒子和荧光粒子2个通道的计数效率评价。首先设计搭建了1套生物气溶胶监测仪计数效率评价装置,然后通过表面原子转移自由基聚合反应(SI-ATRP)制备了单分散、荧光性质稳定的荧光聚苯乙烯微球。采用制备的荧光微球雾化法发尘,模拟生物气溶胶环境,开展计数效率的评价,总粒子数和荧光粒子数计数效率分别为98.9%和98.1%,评价结果表明搭建的装置能满足荧光法生物气溶胶监测仪计数效率的评价需求,对生物气溶胶检测具有意义。

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	潘一廷
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	杨振琪
	商宇扬

关键词 ：计量学, 生物气溶胶, 计数效率, 荧光聚苯乙烯微球, 雾化法

Abstract：Bioaerosol monitor is a kind of new instrument for fast and real time monitoring of air bioaerosol. The study evaluated the counting efficiency of the total particle channel and fluorescent particle channel of bioaerosol monitor instrument. First, a set of evaluation device for counting efficiency of bioaerosol monitor was designed and built. Then, the monodisperse and stable fluorescent polystyrene microspheres were prepared by SI-ATRP method. Bioaerosol environment was generated by atomizing fluorescent particles and found that the counting efficiency of total particle number and fluorescence particle number was 98.9% and 98.1%, respectively. The results showed that the device can meet the needs of the evaluation of the counting efficiency of the fluorescent bioaerosol monitor. It provided a scientific method for systematically evaluating the counting efficiency of a bioaerosol monitor, and the result was of great significance to the detection of bioaerosols.

Key words： metrology bioaerosol counting efficiency fluorescent polystyrene microspheres;atomizing method

收稿日期: 2021-06-11 发布日期: 2023-02-21

PACS:

TB99

基金资助:国家市场监督管理总局科技计划项目(2020MK001)

通讯作者: 张国城(1980-),男,福建永春人,北京市计量检测科学研究院正高级工程师,主要从事化学分析与环境监测仪器的计量检测研究。Email: zhanggc@bjjl.cn E-mail: zhanggc@bjjl.cn

作者简介: 潘一廷(1978-),女,安徽怀宁人,北京市计量检测科学研究院高级工程师,主要从事环境监测仪器的计量检测研究。Email: panyt@bjjl.cn

引用本文:

潘一廷,张国城,霍胜伟,田莹,刘佳琪,杨振琪,商宇扬. 基于荧光法生物气溶胶监测仪计数效率评价及方法研究[J]. 计量学报, 2023, 44(2): 279-283.
PAN Yi-ting,ZHANG Guo-cheng,HUO Sheng-wei,TIAN Ying,LIU Jia-qi,YANG Zhen-qi,SHANG Yu-yang. Research on the Counting Efficiency Evaluation and Method of Bioaerosol Monitoring Instrument Based on Fluorescence Method. Acta Metrologica Sinica, 2023, 44(2): 279-283.

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http://jlxb.china-csm.org:81/Jwk_jlxb/CN/10.3969/j.issn.1000-1158.2023.02.18 或 http://jlxb.china-csm.org:81/Jwk_jlxb/CN/Y2023/V44/I2/279

［11］	田莹, 张国城, 吴丹, 等. 生物气溶胶监测仪的校准方法比较［J］. 计量学报, 2022, 43(1): 140-144.
	隋志伟, 刘晓夏, 王晶, 等. 粘质沙雷氏菌测量方法研究［J］. 计量学报, 2018, 39(4): 588-592.
［2］	Yamakawa M, Kitagawa A, Ogura K, et al. Computational investigation of prolonged airborne dispersion of novel coronavirus-laden droplets ［J］. Journal of Aerosol Science, 2021, 155: 1-15.
［4］	Asadi S, Bouvier N, Wexler A S, et al. The coronavirus pandemic and aerosols: Does COVID-19 transmit via expiratory particles ［J］. Aerosol Science and Technology, 2020, 54(6): 635-638.
	Liu L, Zhou H, Lin B R, et al. Real-time monitoring and controlling strategies of indoor environment in the frontline hospitals during COVID-19 pandemic ［J］. Chinese Science Bulletin, 2021, 66(4): 424-432.
	Choi K, Ha Y, Lee H K, et al. Development of a biological aerosol detector using laser-induced fluorescence and a particle collection system ［J］. Instrumentation Science & Technology, 2014, 42(2): 200-214.
	Hairston P P, Ho J, Quant F R. Design of an instrument for real-time detection of bioaerosols using simultaneous measurement of particle aerodynamic size and intrinsic fluorescence ［J］. Journal of Aerosol Science, 1997, 28(3): 471-482.
［16］	田莹,张国城,李晶晶,等. 生物气溶胶雾化器对大肠杆菌活性影响的评价［J］. 计量学报, 2022, 43(5): 696-700.
	Autofluorescence of atmospheric bioaerosols—fluorescent biomolecules and potential interferences
［5］	刘荔, 周浩, 林波荣, 等. 新型冠状病毒肺炎疫情下隔离医院室内环境安全实时监测与防控策略［J］. 科学通报, 2021, 66(4): 424-432.
［6］	Ma J, Qi X, Chen H, et al. COVID-19 patients in earlier stages exhaled millions of SARS-CoV-2 per hour ［J］. Clinical Infectious Diseases, 2020, 72(10): 1283-1295.
［9］	田莹,张国城,吴丹,等. 生物气溶胶监测仪生物计数评价装置的搭建和方法研究［J］. 计量学报, 2021, 42(12): 1686-1691.
［15］	袁文燕, 王鹏, 伯鑫, 等. 含菌气溶胶扩散对人群的潜在影响风险［J］. 中国环境科学, 2020, 40(7): 3173-3178.
［18］	GB/T 38517-2020颗粒生物气溶胶采样和分析通则［S］.
［3］	Baker R E, Yang W, Vecchi G A, et al. Susceptible supply limits the role of climate in the early SARS-CoV-2 pandemic ［J］. Science, 2020, 369(6501): 315-319.
［8］	Fears A C, Klimstra W B, Duprex P, et al. Persistence of severe acute respiratory syndrome coronavirus 2 in aerosol suspensions ［J］. Emerg Infect Dis, 2020, 26(9): 2168-2171.
［10］	Jeong Y S, Lee J M, Park J, et al. Development of a real-time handheld bioaerosol monitoring system using ultraviolet-light emitting diode induced fluorescence ［J］. Instrumentation Science & Technology, 2020, 48(4): 1-14.
	Tian Y, Zhang G C, Wu D, et al. Comparison of Biological Calibration Methods of Bioaerosol Monitor［J］. Acta Metrologica Sinica,2022,43(1):140-144.
［12］	Sivaprakasam V, Huston A L, Scotto C S, et al. Multiple UV wavelength excitation and fluorescence of bioaerosols ［J］. Optics Express, 2004, 12(19): 4457-4466.
［14］	Benjamin E, Swanson, Alex J, et al. Development and characterization of an inexpensive single-particle fluorescence spectrometer for bioaerosol monitoring ［J］. Optics Express, 2018, 26(3): 3646-3660.
	Tian Y,Zhang G C,Li J J, et al. Evaluation of the Influence of Bioaerosol Atomizers on the Activity of E.coli［J］. Acta Metrologica Sinica, 2022, 43(5): 696-700.
	Sui Z W, Liu X X, Wang J, et al. Study on measurement method of serratia marcescens ［J］. Acta Metrologica Sinica, 2018, 39(4): 588-592.
	Xu A, Xiong C, Zhang P, et al. Research on dual-channel detection technology of bio-aerosols with intrinsic fluorescence measurement ［J］. Acta Optica Sinica, 2013, 33(8): 130-135.
［20］	Sivaprakasam V, Huston A L, Scotto C S, et al. Multiple UV wavelength excitation and fluorescence of bioaerosols ［J］. Optics Express, 2004, 12(19): 4457-4466.
［J］	Atmospheric Measurement Techniques, 2012, 5(1): 37-71.
［7］	Phlker C, Huffman J A, Pschl U. Autofluorescence of atmospheric bioaerosols fluorescent biomolecules and potential interferences ［J］. Atmospheric Measurement Techniques, 2012, 5(1): 37-71.
［13］	Kaliszewski M, Wodarski M, Jarosaw M, et al. A new real-time bio-aerosol fluorescence detector based on semiconductor CW excitation UV laser ［J］. Journal of Aerosol Science, 2016, 100: 14-25.
［19］	徐傲, 熊超, 张佩, 等. 基于本征荧光测量的双通道生物气溶胶检测技术研究［J］. 光学学报, 2013, 33(8): 130-135.
［1］	Mu Y, Shao M, Zhong B, et al. Transmission of SARS-CoV-2 virus and ambient temperature: a critical review ［J］. Environmental Science and Pollution Research, 2021, 28: 37051-37059.
	Tian Y,Zhang G C,Wu D,et al. Device Construction and Method Research of Biological Counting Evaluation of Bioaerosol Detectors［J］. Acta Metrologica Sinica, 2021, 42(12): 1686-1691.
	Yuan W Y, Wang P, Bo X, et al. The potential impact of bioaerosol emissions on human health threats ［J］. China Enviromental Science, 2020, 40(7): 3173-3178.
［21］	Pöhlker C, Huffman J A, Pöschl U.
［17］	Kabir E, Azzouz A, Raza N, et al. Recent advances in monitoring, sampling, and sensing techniques for bioaerosols in the atmosphere ［J］. ACS Sensors, 2020, 5(5): 1254-1267.