Abstract：Single cell sequencing is a new technology which can sequence and analyze the related omics at the level of single cell. It can not only obtain the genetic information of single cell, but also solve the problem of multicellular heterogeneity. In the past decade, single cell research has developed from cell separation, amplification and sequencing to high-throughput single cell sequencing, and many new and efficient methods and platforms have emerged. The development process of single cell sequencing is reviewed. The advantages, disadvantages and applicability of different methods and commercial platforms are discussed, and the impact of single cell sequencing on biometrology is prospected.
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