Abstract：In order to develop plasmid nucleic acid reference material of porcine reproductive and respiratory syndrome virus (PRRSV), a digital PCR method was established, and several laboratories were combined to conduct cooperative evaluation of the reference material by using the digital PCR method. The factors affecting the accuracy of plasmid evaluation in the process of cooperative evaluation were discussed, and the uncertainty of the reference material was evaluated. The two reference materials have been certified with codes GBW(E)091038 and GBW(E)091039, which can provide quantitative standards for detection methods and can be used in method evaluation, product quality control and many other aspects.
牛春艳,张永卓,杨佳怡,董莲华,傅博强,王晶. 基于数字PCR的质粒核酸标准物质合作定值研究[J]. 计量学报, 2021, 42(11): 1522-1527.
NIU Chun-yan,ZHANG Yong-zhuo,YANG Jia-yi,DONG Lian-hua,FU Bo-qiang,WANG Jing. Resrach on the Collaborative Value Assignment of Plasmid Nucleic Acid Refernce Materials Based on Digital PCR. Acta Metrologica Sinica, 2021, 42(11): 1522-1527.
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2021, Vol. 42 
