液相色谱串联质谱法测定鸡蛋中利巴韦林及其代谢物总残留的不确定度评定

doi:10.3969/j.issn.1000-1158.2021.09.21

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摘要为提高鸡蛋食品安全检测结果准确性,建立了一种液相色谱串联质谱法(liquid chromatography-tandem mass spectrometry, LC-MS/MS)测定鸡蛋中的利巴韦林及其代谢物总残留量的不确定度数学模型。依据SN/T 4519—2016《出口动物源食品中利巴韦林残留量的测定》中的方法,对整个检测过程进行了不确定度评定。不确定度分量主要来源有测量的重复性、回收率、样品的称量、样品前处理稀释和定容、标准溶液配制、标准曲线拟合及仪器测定引入。经评定鸡蛋中利巴韦林及其代谢物总残留量结果为X=(9.94±0.88)μg/kg, k=2; 其中标准曲线拟合过程中引入的不确定度分量影响最大。

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	李德恒
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	李高天

关键词 ：计量学, 利巴韦林, 液相色谱串联质谱法, 不确定度, 鸡蛋安全检测

Abstract：In order to improve the accuracy of egg safety inspections test results, the uncertainty determination of total residues of ribavirin and its metabolites in eggs was evaluated by LC-MS/MS. According to the method of national standard SN/T 4519—2016 "Determination of ribavirin residue in foodstuffs of animal origin for export LC-MS/MS methods", the whole experimental process was evaluated. The main sources of uncertainty in evaluation process were the measurement repeatability, recovery rate, the weighting of sample, dilution and constant volume in sample pretreatment, preparation of standard solution, the standard curve fitting process and instrumental analysis. The extended uncertainty was 0.88μg/kg(k=2) when the total residues of ribavirin and its metabolites in eggs was 9.94μg/kg, the standard curve fitting process contributed the most to the uncertainty of measurement result.

Key words： metrology ribavirin LC-MS/MS uncertainty egg safety inspections

收稿日期: 2020-08-23 发布日期: 2021-09-24

PACS:

TB99

通讯作者: 李高天(1990-),河北赵县人,杭州市食品药品检验研究院工程师,研究方向为食品药品检验与研究。Email:yaer19905230@sina.com E-mail: ruanjz1028@163.com

作者简介: 李德恒(1966-),海南儋州人,海南西部中心医院中西医结合内科副主任医师,研究方向为中西医结合感染性疾病与肝胆病的临床研究。Email:592704906@qq.com

引用本文:

李德恒,陆晶芳,曾金林,尹丹韩,李高天. 液相色谱串联质谱法测定鸡蛋中利巴韦林及其代谢物总残留的不确定度评定[J]. 计量学报, 2021, 42(9): 1250-1256.
LI De-heng,LU Jing-fang,ZENG Jin-lin,YIN Dan-han,LI Gao-tian. Evaluation of Uncertainty in Determination of Total Residues of Ribavirin and its Metabolites in Eggs by LC-MS/MS. Acta Metrologica Sinica, 2021, 42(9): 1250-1256.

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http://jlxb.china-csm.org:81/Jwk_jlxb/CN/10.3969/j.issn.1000-1158.2021.09.21 或 http://jlxb.china-csm.org:81/Jwk_jlxb/CN/Y2021/V42/I9/1250

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