Development of Glucose in Human Serum Reference Material
LI Wu-rong1,2,YANG Bin3,HUANG Yan-jie1,ZHANG Ning3,LUO Si-ting1, WANG Jing3,WU Quan-zhou2,WU Li-Qing3
1. Guangdong Provincial Key Laboratory of Modern Geometric and Mechanical Metrology Technology, Guangdong Provincial Institute of Metrology,Guangzhou, Guangdong 510405, China
2. School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine, Guangzhou, Guangdong 510006, China
3. Center for Advanced Measurement Science (Division of Life Science Metrology), National Institute of Metrology, Beijing 100029, China
Abstract：Blood glucose is a direct indicator for diagnosis and discrimination of diabetes, and reference materials are important guarantee for the accuracy and comparability of blood glucose detection results.The process of developing glucose in human serum reference materials with six different concentration levels includes the preparation, value determining, uniformity, stability test, uncertainty evaluation and interoperability evaluation of reference materials. Two different principles of isotope dilution liquid chromatography tandem mass spectrometry and isotope dilution gas chromatography mass spectrometry were used to determine the concentration value of glucose in human serum, and the differences and causes between the analysis results of the two methods were analyzed. The quantitative value of glucose in human serum reference material at different concentration levels in human serum covers 1.70~28.12mmol/L. This series of reference material have good uniformity and stability, and have obtained the gradation certificate of national secondary certified reference material, the number is GBW (E) 091140~GBW(E) 091145, which are expected to be used in clinical tests and related areas for quality evaluation of measurement of serum glucose items and other aspects.
Xu C X, Wang Y P, An Z Y, et al. New arctic marine sediment certified reference material for elemental analysis［J］. Analytical Letters, 2019, 52(3): 401-410.
Chen L, Huang Y J, Zhou J Y, et al. Equivalent Simulation and Reference Material of the Bacterial Colonies［J］. Acta Metrologica Sinica, 2020, 41(1): 121-128.
Zhou J Y, Huang Y J, Xu J B, et al. Development of Acid Orange II Purity Reference Material［J］. Acta Metrologica Sinica, 2020, 41(9): 1163-1170.
Liu W X, Luo Y, Yang W C, Applications of Organic Isotope Dilution Mass Spectrometry in Food Safety Analysis［J］. Chemical World, 2011, 52(3): 184-187, 177.
Qiu F Y, Ma M. Comparison of the Glucose Oxidase-Oxygen Rate Method with the Other Method in Determining Blood Sugar［J］. Natural Science Journal of Hainan University, 2004, 22(3): 266-268.
［10］
Khalil O S. Spectroscopic and clinical aspects of noninvasive glucose measurements［J］. Clinical Chemistry, 1999, 45(2): 165-177.
［11］
Hanne S U, Lundblad A. Accurate and precise isotope dilution mass spectrometry method for determining glucose in whole blood［J］. Clinical Chemistry, 1997, 43(5): 794-800.
Quan C, Wu L Q, Dai X H, et al. CCQM-K11. 1 International Comparison on the Determination of Serum Clucose［J］. Chemical Analysis and Meterage, 2006, 15(4): 50-53.
Zhang Y, Zhao S, Lu S, et al. Study on Intervention of Blood Sugar and Blood Lipid in Diabetic Mice by Abrus Cantoniensis and Armeniaca mume Sieb and Their Compatibility［J］. TMR Pharmacology Research, 2021(4): 1-7.
Zhang T J. Zhang C B, Zhang J T, et al. Determinafion of serum glucose by isotope dilution liquid chromatography tandem mass spectrometry［J］. Chinese Journal of Laboratory Medicine, 2010, 33(1): 75-80.
Liu Y Y, Zhang L, Yang Q S. Development of Reference Material for White Blood Cell Count in Urine Sediment［J］. Acta Metrologica Sinica, 2022, 43(3): 427-432.
Ding Z T, Quan C, Jin J S, et al. Determination of serum glucose by gas chromatography-mass spectrometry［J］. Journal of Beijing University of Chemical Technology(Natural Science Edition), 2010, 37(2): 109-112.
2023, Vol. 44 
