Abstract：The mass balance method is a classic method to determine the purity of organic reference materials. The GBW(E)100373 Acid Orange II purity reference material was developed by the mass balance method, and the strict procedures of preparation, homogeneity test, stability test, value assessment, uncertainty evaluation, comparison and verification of reference materials were introduced. Finally, a purity reference material of acid orange II with certified purity value of 99.2% and relative expanded uncertainty of 1.0% (k=2) was obtained, which can play an important role in the fields of instrument calibration and method evaluation in food and cosmetic analysis.
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2020, Vol. 41 
