Abstract：The static weighing method and optical method are used to measure the ultramicro volume. In the optical method, the measurement systems based on the time-domain and spatial-domain spectroscopic methods are designed and built respectively. The measurement data of the two optical measurement systems at 1 μL and 10 μL are 0.967 μL and 0.929 μL, and 9.976 μL and 9.729 μL, respectively The renaturation is 3.2% and 2.54%, and the spatial spectroscopic measurement system is 3.6% and 2.02%. The same conclusion is obtained with the static measurement method, which proves the feasibility of optical method in the field of ultramicro volume measurement. Compared with the two optical systems, the detector sensitivity of the time-domain spectrometer is better, and more light intensity information can be detected in the ultra-fine volume measurement of low concentration; Compared with the static weighing method,.the optical method for liquid evaporation is simpler, and the design of optical elements can effectively reduce the influence of evaporation.
［1］王金涛, 陈超云, 李志昊, 等.超微量液体容积双波长参比测量方法研究［J］. 计量学报, 2015, 36(3): 256-259.
Wang J T, Chen C Y, Li Z H, et al. Study on Dualwavelength Photometric Method for Ultra Micro Liquid Volume Measurement［J］. Acta Metrologica Sinica, 2015, 36(3):256-259. ［2］周湘平. 小型全自动生化分析仪微量移液系统关键技术研究［D］. 天津: 天津工业大学, 2018. ［3］张诚春, 许诚, 张怡雯. 基于光度原理的移液器校准方法研究［J］. 计量与测试技术, 2018, 45(10): 56-59.
Zhang C C, Xu C, Zhang Y W. Study on Calibration Method for Locomotive Pipette Based on Photometric Principle［J］. Metrology & Measurement Technique, 2018,45(10): 56-59. ［4］万磊. 计量工作在医疗设备质量管理中的重要作用［J］. 中国医学装备, 2017, 14(3): 131-133. ［5］赵玉晓, 谢玄达, 孙斌,等. 移液器容量自动校准系统研究［J］. 计量学报, 2019, 40(3): 392-396.
Zhao Y X, Xie X D, Sun B, et al. Research on automatic calibration system of pipette［J］. Acta Metrologica Sinica, 2019, 40(3): 392-396. ［6］李建明, 龙永疆, 李加新. 应用γ计数法校准移液器［J］. 新疆医学, 2007(5): 78-79.
Li J M, Long X J, Li J X. Calibration of pipette with gamma counting method［J］. Xinjiang Medical Journal, 2007(5): 78-79. ［7］JJG 646—2006移液器检定规程［S］. 2006. ［8］施娟. 基于衡量法的移液器校准技术研究［J］.计量与测试技术, 2017, 44(8): 113-115.
Shi J. Study on Pipette Calibration Based on Gravimetric Methods［J］. Metrology & Measurement Technique,2017,44(8): 113-115. ［9］李仲贤. 探讨水分蒸发现象对移液器检定数据的影响［J］. 计量与测试技术, 2019,46(2): 41-43.
Li Z X. Discussion on the influence of water evaporation on the verification data of pipette ［J］. Metrology & Measurement Technique, 2019,46(2): 41-43. ［10］郝丑怡. 常见光谱仪的原理分析及性能比较［J］. 山西师范大学学报(自然科学版), 2013, 27(S2): 50-53.
Hao C Y. Principle analysis and performance comparison of common spectrometers［J］. Journal of Shanxi Normal University (Natural Science Edition), 2013, 27(S2): 50-53. ［11］徐丹阳. 多功能光栅光谱仪的研制［D］. 杭州: 浙江工业大学, 2010. ［12］杨增鹏, 唐玉国, 巴音贺希格, 等. 小型高光谱分辨率光栅单色仪的研制［J］. 光谱学与光谱分析, 2016, 36(1): 273-278.
Yang Z P, Tang Y G, Bayanheshig et al. Research on Small-Type and High-Spectral-Resolution Grating Monochromator［J］. Spectroscopy and Spectral Analysis, 2016,36(1): 273-278. ［13］陈建军. 棱镜-光栅型短波红外成像光谱仪关键技术研究［D］. 长春: 中国科学院大学(中国科学院长春光学精密机械与物理研究所), 2019. ［14］胡长宏, 李玉, 王丽红, 等. 利用光栅光谱仪测量氘灯、溴钨灯的发射光谱［J］. 物理与工程, 2014(S1): 87-88.
Hu C H, Li Y, Wang L H,et al. Measurement of emission spectra of deuterium lamp and tungsten bromide lamp by grating spectrometer［J］. Physics and Engineering, 2014(S1): 87-88. ［15］余明. 基于光电检测技术的微弱光谱采集系统设计与研究［D］. 广州: 广东工业大学, 2015. ［16］王毅. 便携式拉曼光谱仪及数据处理关键技术研究［D］. 成都: 电子科技大学, 2019.
2022, Vol. 43 
