生物气溶胶雾化器对大肠杆菌活性影响的评价

doi:10.3969/j.issn.1000-1158.2022.05.21

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摘要生物气溶胶的研究依赖于稳定可靠的雾化过程。从颗粒数量浓度、粒径大小分布、细菌可培养性和细胞膜破损等方面评价不同气溶胶发生器的性能, 并探讨进气流量的影响。结果表明在气溶胶发生过程中的机械应力严重损害细胞膜的完整性,使得细菌的可培养率降低90%以上,并且随着进气流量的增加,细胞碎片的比例增加,细胞膜损伤更为严重。由于生物气溶胶的研究不仅需要高浓度的微生物,更要保证细胞的完整性和可培养性,因此以上研究将对生物气溶胶发生器的性能评价和选择提供依据。

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	田莹
	张国城
	李晶晶
	刘佳琪
	潘一廷
	吴丹
	沈上圯
	霍胜伟

关键词 ：计量学, 生物气溶胶雾化器, 实时定量PCR, 细胞膜完整性, 细菌可培养性

Abstract：The research of bioaerosol relies on a stable and reliable atomization process. The performances of different aerosol generators are evaluated from the aspects of particle concentration, particle size distribution, bacterial culturability and cell membrane damage, and the influence of inlet flow is discussed.The results show that the mechanical stress in the process of aerosol generation seriously damages the integrity of the cell membrane, which reduces the cultivable rate of bacteria by more than 90%, and with the increase of air flow, the proportion of cell debris increases, and the cell membrane damage becomes more serious. Since the research of bioaerosol not only requires high concentrations of microorganisms, but also ensures the integrity and cultivability of cells, the above study will provide a basis for the performance evaluation and selection of bioaerosol generators.

Key words： metrology bioaerosol atomizer real-time quantitative PCR cell membrane integrity bacterial culturability

收稿日期: 2021-07-19 发布日期: 2022-05-18

PACS:

TB99

基金资助:国家市场监督管理总局科技计划项目(2021MK003)

通讯作者: 张国城(1980-),男,福建永春人,北京市计量检测科学研究院教授级高工,主要从事化学分析与环境监测仪器的计量检测研究。Email:zhanggc@bjjl.cn E-mail: zhanggc@bjjl.cn

作者简介: 田莹(1990-),女,河南焦作人,北京市计量检测科学研究院工程师,主要从事颗粒物相关环境监测仪器研究。Email:tiany@bjjl.cn

引用本文:

田莹,张国城,李晶晶,刘佳琪,潘一廷,吴丹,沈上圯,霍胜伟. 生物气溶胶雾化器对大肠杆菌活性影响的评价[J]. 计量学报, 2022, 43(5): 696-700.
TIAN Ying,ZHANG Guo-cheng,LI Jing-jing,LIU Jia-qi,PAN Yi-ting,WU Dan,SHEN Shang-yi,HUO Sheng-wei. Evaluation of the Influence of Bioaerosol Atomizers on the Activity of E.coli. Acta Metrologica Sinica, 2022, 43(5): 696-700.

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http://jlxb.china-csm.org:81/Jwk_jlxb/CN/10.3969/j.issn.1000-1158.2022.05.21 或 http://jlxb.china-csm.org:81/Jwk_jlxb/CN/Y2022/V43/I5/696

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