基于时域分光法的微小容量测量方法研究

doi:10.3969/j.issn.1000-1158.2021.09.10

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摘要为了实现微小容量的在线测量,研究了一种基于时域分光法的微小容量检测方法。该方法基于溶液吸光度与溶液浓度的关系定理,通过双波长测量减小了实验耗材的加工误差影响。基于时域分光的模式,采用光栅分光将复色光源在时间域上分离出单色光后由探测器检测溶液吸光度变化。最后计算出溶液体积,同时可以通过配制不同浓度的溶液实现多次测量,提高实验耗材的利用率。在100μL和50μL的液体容量测量值分别为99.67μL和50.11μL,重复性为0.35%和1.22%,与传统的重力法进行比较获得了同样的结论,证明了基于时域分光法的微小容量测量方法的可行性。

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	刘鑫行
	张殿龙
	张竟月
	孙斌
	佟林
	赵玉晓
	钟家栋

关键词 ：计量学, 微小容量, 时域分光, 双波长测量

Abstract：In order to adapt to the current situation of the application of micro capacity and realize the on-line measurement of micro capacity, a micro capacity detection method based on time-domain spectrophotometry is studied. Based on the relationship between the absorbance of solution and the concentration of solution, this method reduces the influence of the processing error of the experimental consumables through the dual wavelength measurement. Based on the time-domain spectroscopic mode, the grating spectrophotometer is used to separate the monochromatic light from the polychromatic light source in the time domain, and then the detector detects the change of the absorbance of solution. Finally, the volume of solution is calculated. At the same time, the actual solution with different concentration can be prepared many times of measurement, to improved the utilization rate of experimental consumables. At 100μL and 50μL, the measurement value of liquid capacity is 99.67μL and 50.11μL, respectively, and the repeatability is 0.35% and 1.22%. Compared with the traditional gravity method, the same conclusion is obtained, which proves the feasibility of the micro capacity measurement method based on time-domain spectrophotometry.

Key words： metrology micro capacity time domain light splitting dual wavelength measurement

收稿日期: 2019-12-30 发布日期: 2021-09-24

PACS:

TB938.3

通讯作者: 孙斌(1972-),男,黑龙江勃利人,中国计量大学教授,博士,主要从事容流量计量、温湿度计量、过程参数检测与控制方面的研究。Email: bsun555@cjlu.edu.cn E-mail: bsun555@cjlu.edu.cn

作者简介: 刘鑫行(1996-),男,浙江金华人,中国计量大学在读硕士研究生,主要研究方向为微小容量测量。Email:996396697@qq.com

引用本文:

刘鑫行,张殿龙,张竟月,孙斌,佟林,赵玉晓,钟家栋. 基于时域分光法的微小容量测量方法研究[J]. 计量学报, 2021, 42(9): 1182-1186.
LIU Xin-xing,ZHANG Dian-long,ZHANG Jing-yue,SUN Bin,TONG-Lin,ZHAO Yu-xiao,ZHONG Jia-dong. Research on the Method of Small Capacity Measurement Based on Time Domain Spectrophotometry. Acta Metrologica Sinica, 2021, 42(9): 1182-1186.

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