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2025 Vol. 46, No. 2
Published: 2025-02-28
 
151 Traceable-Grating-Based High Precision Displacement Measurement System and Its Uncertainty Analysis
GAO Qiyuan, SHI Yushu, ZHANG Shu, SHI Zhoumiao, WANG Kang, HU Jiacheng
DOI: 10.3969/j.issn.1000-1158.2025.02.01
By constructing a traceable grating displacement measurement system with the grating pitch calibration value as the value of the pitch， the transfer of the value from the meter definition to the grating displacement measurement system was achieved. The influence of the alignment error between the grating and the read head on the grating pitch and interference signal contrast was analyzed. The static stability experiment of the system and the displacement measurement consistency experiment with the laser interferometer were conducted， and the experimental results showed that： The short-term stability of the traceable grating displacement measurement system was 3.35 nm， and the long-term stability was better than 70 nm； The system and the laser interferometer have good consistency in the measurement results of different stroke displacements， at each measurement point within the 10 μm and 500 μm displacement ranges， the absolute values of the measurement average errors do not exceed 0.07% and 0.013% respectively. Finally， the main sources of system measurement uncertainties were analyzed， and the system uncertainty for 10 μm displacement measurements was evaluated， the relative expanded uncertainty result was 0.83% （k = 2）.
2025 Vol. 46 (2): 151-159 [Abstract] ( 23 ) HTML (1 KB)  PDF  (0 KB)  ( 2 )
160 Study on Relative Spectral Responsivity Measurement and Drift Correction of MCT Detector
WANG Qiwen, ZHAO Yunlong, AN Baolin, LU Xiaofeng, Yang Chengzhi, WANG Gao, DONG Wei, LI Yangjun
DOI: 10.3969/j.issn.1000-1158.2025.02.02
As a key component of photodetector， accurate measurement of the responsivity of MCT detector is very important to ensure the detection accuracy of the system. However， the detector responsivity will drift with temperature changes， material aging， and fluctuations in environmental conditions， affecting the reliability of the measurement results. Most of the existing research focuses on the wavelength response characteristics of MCT detectors， but the research on the drift problem is relatively weak. Using pyroelectric detector as the standard detector， the relative spectral responsivity measurement system of photodetector was built. The relative spectral responsivity of MCT detector in 3.5~5 μm was measured and compared with the existing results. The repeatability of the pyroelectric detector signal was measured experimentally by repeatedly switching the pyroelectric detector and MCT detector. The drift characteristics of MCT detector with time in a typical period of relative spectral responsivity measurement were studied， and a sequence cross measurement method was proposed to correct the quasi-linear drift effect. The relative standard deviation of the relative spectral responsivity measurement results of MCT detectors was reduced from 0.74% to 0.38%. The use of linear correction coefficients for correction is more suitable for measuring the relative spectral responsivity of fast MCT detectors.
2025 Vol. 46 (2): 160-166 [Abstract] ( 16 ) HTML (1 KB)  PDF  (0 KB)  ( 1 )
167 Research on Vehicle Infrared Target Detection Improved Algorithm Based on YOLOv8
HOU Jun, YANG Jie, SHAO Kaiqing
DOI: 10.3969/j.issn.1000-1158.2025.02.03
Aiming at the problem of mutual occlusion of targets and missed detection of small-scale targets in vehicle-mounted infrared image detection， an vehicle-mounted infrared target detection improved algorithm（VITD-YOLO） based on YOLOv8 is proposed. Firstly， a large-size feature network prediction layer（S-layer）， is added to the Neck network to enhance the detection accuracy of the network for small objects. Secondly， the C2F-DA module is designed in Backbone network， and the offset lightweight structure is used to enhance the perception ability of the model to the local features of the target. A dynamic convolutional head（Dy-head） detection module is designed by combining three different scales of self-attention to improve the location and classification accuracy of occluded and dense targets. Finally，Focal-SIoU is used as the loss function of the network to solve the problem of class imbalance of pedestrian and vehicle targets in the training samples and improve the training and reasoning ability of the network. The algorithm is tested on FLIR infrared data set. Experimental results show that VITD-YOLO has good detection effect and robustness and higher accuracy for small-scale target detection. The average precision of the algorithm reaches 91.2%，which is 2.5% higher than that of the original algorithm， and the recall rate reaches 83.4%， which is 3.2% higher than that of the original algorithm.
2025 Vol. 46 (2): 167-176 [Abstract] ( 28 ) HTML (1 KB)  PDF  (0 KB)  ( 2 )
177 Multi-objective Optimization Design Method of Helical Gear Flowmeter
HAO Ning, WANG Yongfeng, ZHU Fengzhang, LIU Zikang, WANG Xue, WANG Lintao
DOI: 10.3969/j.issn.1000-1158.2025.02.04
Aiming at the problem that the existing design methods cannot effectively improve the comprehensive performance of helical gear flowmeter（HGF）， the multi-objective optimization design method of HGF is studied. Through multidisciplinary modeling， the theoretical models of flow pulsation，internal leakage and viscous friction power loss of HGF are obtained. Then， the influence of each design variable on the main performance indicators of HGF is analyzed by design of experiments. Finally， the flow pulsation and friction power loss are selected as the optimization objectives， and the measurement accuracy of the flowmeter is used as the constraint， and NSGA-II is used to solve the multi-objective optimization problem. The calculation results show that the optimized HGF flow pulsation is reduced by 31%， and the friction power loss is reduced by 18.4%.
2025 Vol. 46 (2): 177-183 [Abstract] ( 20 ) HTML (1 KB)  PDF  (0 KB)  ( 4 )
184 Design and Study of Movable Cone for Variable Area Orifice Flowmeter
ZHOU Yan, XU Kuangyu, ZHU Ning, LI Ning
DOI: 10.3969/j.issn.1000-1158.2025.02.05
Through the study of variable area orifice flowmeter， it is found that the surface shape of the active cone is a key factor in the linearity and range ratio of the flowmeter. A new cone-shaped is designed，which makes the flow rate of the variable area orificeplate flowmeter linear with the differential pressure.A functional relation of the surface curve of a new cone-shaped is derived，the main influencing factors are the surface machining accuracy of the cone and the β value of the flow meter. The linear relationship between flow rate and differential pressure between a new cone-shaped and a regular conical body is compared，and experiments show that the linear range of the new cone-shaped is wider， and its range ratio is greater than 150∶1.
2025 Vol. 46 (2): 184-189 [Abstract] ( 18 ) HTML (1 KB)  PDF  (0 KB)  ( 1 )
190 Variable Angle Performance Prediction of a Slanted Axial-flow Pump Based on HBA-SVR Model
ZHENG Haisheng, ZHOU Peijian, XIAO Gang, MOU Jiegang, XIANG Chun, QIAN Heng
DOI: 10.3969/j.issn.1000-1158.2025.02.06
To address the difficulty and cost associated with obtaining performance curves for different blade angles， a performance prediction method for slanted axial-flow pump based on the hybrid HBA-SVR model is proposed. The standard bat algorithm is enhanced with directional acceleration and variation strategies to optimize the SVR. The model is trained by using the operating data of the 30° slanted axial-flow pump， and applied to the variable angle performance prediction of the slanted axial-flow pump. The average relative errors for head and efficiency are reduced to 1.49% and 0.41% respectively， with convergence times of 15.47 s and 18.78 s. When compared to the results of standard bat optimization support vector regression prediction， the convergence times are reduced by 122.11% and 103.62% respectively. Moreover， compared to PSO， GA， and BA optimized SVR， the head prediction errors are reduced by 29.53%， 70.46%， and 131.54% respectively， and the efficiency prediction errors are reduced by 7.31%， 9.75%， and 19.51% respectively. The results indicate that the proposed model effectively predicts the variable angle performance of slanted axial-flow pump.
2025 Vol. 46 (2): 190-197 [Abstract] ( 30 ) HTML (1 KB)  PDF  (0 KB)  ( 1 )
198 Research on Absorbance Monitoring Micro Liquid Volume Measurement System and Method Based on Lambert-Beer Law
CHANG Xu, WANG Jintao, LIU Xiang, ZHANG Jingyue, TONG Lin, GUO Ligong, XU Changhong, SHI Wencai, XU Haoming
DOI: 10.3969/j.issn.1000-1158.2025.02.07
To overcome the shortcoming of hydro-weighing method，which has high environmental requirements， liquid evaporation affects accuracy and is difficult to realize field detection， a micro liquid volume measurement method was studied based on Lambert-Beer. To improve measurement repeatability， a measurement system with absorbance monitoring optical path was designed to monitor interference factors such as light source fluctuations，and a correction coefficient was proposed. A measurement scheme was designed，and corresponding mathematical analytical model was proposed to calculate micro liquid volume. A practical measurement system was built and experiments were carried out. The liquid mixing experiment verified that the measurement efficiency was improved. The measurement experiments carried out on the volume detection point of 100 μL verified that the absorbance monitoring optical path can improve the repeatability from 2.02% to 0.76%. The final measurement results meet the requirements of JJG 646-2006， which verifies the effectiveness of the measurement system.
2025 Vol. 46 (2): 198-203 [Abstract] ( 19 ) HTML (1 KB)  PDF  (0 KB)  ( 2 )
204 Research on Metrology Calibration Technology for the Multi-points Pasted Strain-gauges System On-line
ZHOU Ning, FENG Jianmin, SONG Hao, YAO Jianping, ZHAO Qidi, ZHANG Huijun, HUANG Xiaolong, YIN Wei, GAO Zhanpeng, WU Jun, YU Chong
DOI: 10.3969/j.issn.1000-1158.2025.02.08
In the static strength test of the whole structure of a large passenger aircraft, a technical idea of establishing a pseudo-traceability chain by reducing the dispersion of sensitive components was proposed to address the on-line calibration problem of multi-point adhesive strain measurement system. The tentative research was developed， and an improvement was got on the drop of dispersion to 1% and 2.8% separately for FBG and metal strain-gauges. To research and develop a strain standard， and to study measurement results and their tracing methods of FBG，strain gauge and photogrammetry， a practical way was found at last. That can build a true-traceability chain for pasted strain-gauge sensors by non-touch instrument with high precision， in addition to the low dispersion of strain sensors. It could be expected to operate calibration-on-line at multi-point strain gauge site. Moreover， a new kind of automatic calibration equipment was developed for large-scall signal channels of pasted strain-gauge sensors， and total efficiency was raised to 10 times.
2025 Vol. 46 (2): 204-212 [Abstract] ( 22 ) HTML (1 KB)  PDF  (0 KB)  ( 2 )
213 Kinematic Calibration of the 6-DOF Parallel Robot Driven by Linear Motor
ZHANG Xinlu, LIU Zhihua, CAI Chenguang, KONG Ming, FU Lei
DOI: 10.3969/j.issn.1000-1158.2025.02.09
To tackle slow iterative convergence in the optimization algorithm caused by inconsistent position and attitude dimensions in kinematic calibration of parallel robots， a method for unifying the order of magnitude of displacement and attitude functions in error models is proposed. First， taking the 6-PSS parallel robot driven by a linear motor as the research object， based on the closed-loop vector equation， a dimensionless kinematic parameter error model is constructed by reducing the loss function values of the displacement function and attitude function in the error Jacobian matrix. Second， the least squares algorithm is used to identify the kinematic parameter errors including the spherical hinge point， rod length， and guide rail direction vector of the dynamic and static platform， and the parameters of the control model are further modified based on the identification results. At last， a linear motor-driven 6-PSS parallel robot kinematics calibration experiment is conducted， and the AICON binocular stereo vision device is placed in an indoor environment without sudden changes in light to measure the actual position and attitude of the parallel robot’s end effector. The theoretical position and attitude are compared to obtain the error， and then the accuracy of the prototype is compensated. The experimental results show that after kinematic calibration， the position accuracy of the prototype is improved by 96.736%， the attitude accuracy is improved by 83.750%， and the accuracy compensation is effective， which verifies the effectiveness of the error model.
2025 Vol. 46 (2): 213-221 [Abstract] ( 20 ) HTML (1 KB)  PDF  (0 KB)  ( 2 )
222 Bearing Fault Diagnosis Method Based on Multi-branch Feature Fusion Attention Mechanism
GUO Haiyu, YU Wei, ZHANG Xiaoguang, LU Fanfan, CHEN Yang, ZHAO Xueyi
DOI: 10.3969/j.issn.1000-1158.2025.02.10
A multi-branch multi-scale convolutional neural network with channel attention （MBSACNN） method is proposed to enhance feature extraction and improve accuracy with noises in bearing fault diagnosis.Different from the traditional methods， where only one dimension fault feature was considered， multi-channel multi-input is constructed to extract multi-dimension abundant features and enhance the diversity of sample information from the wavelet transform time-frequency signal， which are combined by the channel attention mechanism.Higher diagnosis accuracy and better noise-robustness are obtained by the MBSACNN compared with the traditional methods， which is verified by Case Western Reserve University （CWRU） bearing dataset and real-world application in cement industry.In the case of a bearing dataset， the accuracy of zero noise and strong noise is 99.99% and 96.97% respectively.Under strong noise，the accuracy of three kinds of cement equipment are all above 97.25%.
2025 Vol. 46 (2): 222-232 [Abstract] ( 24 ) HTML (1 KB)  PDF  (0 KB)  ( 1 )
233 Hydrophone Sensitivity Calibration System under Aerostatic Pressure
LI Bin, WANG Yuebing, ZHAO Peng, XIE Jianyu, CAI Yue
DOI: 10.3969/j.issn.1000-1158.2025.02.11
Aiming at the sensitivity calibration of hydrophones in air， the calibration principle of hydrophone sensitivity under aerostatic pressure is described， and a convenient calibration system is designed and built. Calculate and analyze the variation relationship of air static pressure in the calibration cavity， and control the change of sound pressure in the device to calibrate the sensitivity of hydrophone at a frequency of about 6 Hz. The rationality of the system design is analyzed through simulation and theoretical calculation. The experimental results show that the maximum deviation of the hydrophone sensitivity measured by the calibration system from that measured by the very low-frequency hydrostatic excitation method is not more than 0.5 dB， and the deviation is within 0.6 dB and 1 dB respectively compared with that measured by the vibrating liquid-column method and the standing-wave-tube comparison method with an extended uncertainty of the system of U≈0.8 dB （k=2）， which verifies the feasibility of this method for calibrating the low-frequency sensitivity of the hydrophone under the aerostatic pressure. The feasibility of the method is verified.
2025 Vol. 46 (2): 233-239 [Abstract] ( 24 ) HTML (1 KB)  PDF  (0 KB)  ( 2 )
240 Measurement of Source Level of Low-frequency Hydroacoustic Transducers Based on Laser Vibrometry
WANG Wenxia, QIANG Chenghu, WANG Min, YANG Ping, AI Hua, WANG Ke, HE Longbiao
DOI: 10.3969/j.issn.1000-1158.2025.02.12
Aiming at the problems that the measurement of the radiation source level of low-frequency underwater acoustic transducers relies on large-scale free-field water areas and has high implementation costs， a source level measurement method based on scanning laser vibrometry is proposed. The principle of vibration velocity measurement using heterodyne interferometry and the far-field sound pressure calculation method based on the Rayleigh integral are elaborated. A scanning measurement device for the surface vibration velocity of the transducer is set up and and complex vibration modes on the transducer surface are observed in the experiments. The far-field sound pressure is deduced from the surface vibration and compared with results in the free-field lake test. The differences in the measured source levels at frequencies of 100 Hz and 5 kHz are -2.2 dB and 0.9 dB respectively， and the results are basically consistent， verifying the effectiveness of this method.
2025 Vol. 46 (2): 240-246 [Abstract] ( 19 ) HTML (1 KB)  PDF  (0 KB)  ( 1 )
247 Analysis of the sensitivity characteristics and structural optimization of dual-plane array electrostatic sensor
To address the issues of low sensitivity and uneven sensitive field distribution in the arrayed electrostatic sensor for online monitoring of abrasive particles in aviation engine lubricating oil, a comprehensive optimization of the sensor structure units was conducted through a combination of theoretical analysis and simulation. A novel double-plane 32-plate arrayed electrostatic sensor was designed, and the theoretical model of the sensor was established based on the point charge concept. The influences of plate thickness, coverage angle, length, and double-plane spacing on the sensor's sensitivity were investigated. Subsequently, the sensor configuration was optimized, and corresponding experimental samples were prepared. An experimental platform was set up for testing. The results indicated that the double-plane spacing L and plate length H were crucial factors in enhancing the sensor's sensitivity. The optimized sensor showed a 75.80% increase in sensitivity and a 56.05% improvement in uniformity. Experimental findings were consistent with the trends observed in finite element simulations. When L increased from 1 cm to 2 cm, the monitored voltage amplitude of the electrostatic sensor increased from 29.23 mV to 40.16 mV. Similarly, when H increased from 2 cm to 4 cm, the voltage amplitude increased to 76.43 mV. Further optimization of both L and H resulted in a voltage amplitude increase to 80.77 mV.
2025 Vol. 46 (2): 247-258 [Abstract] ( 11 ) HTML (1 KB)  PDF  (0 KB)  ( 2 )
259 Experimental Study on Calibration Method of Offsite Optical Frequency Comb
XI Wenfei, MENG Fei, LIN Baike, SU Yabei, XUE Xiaobo, ZHAO Yao, GAO Yesheng, CAO Shiying, LIN Yige, LANG Liying, FANG Zhanjun
DOI: 10.3969/j.issn.1000-1158.2025.02.14
To achieve offsite calibration of optical frequency combs， a calibration method using a frequency-stabilized laser as a transfer standard was proposed and experimentally demonstrated. The experiment used a standard optical frequency comb in National Institute of Metrology to calibrate the mean frequency of the transfer laser to 194 369 489 387 885 Hz， with the relative expanded uncertainty of 1.03 × 10-13 （k=2）， the average stability was 1.7×10-13@1 s. The experiment lasted one and a half years and involved several long-distance transports. On this basis， the experiment was calibrated by means of a transfer laser on two off-site optical frequency combs， respectively， in which the measured mean value of the optical frequency comb using a reference to the H-maser is 194 369 489 387 827 Hz， with the relative extended uncertainty of 3.2 × 10-13 （k = 2）， a calibration value of +58 Hz， and an average stability of 2.0 × 10-13@1s； The measured mean value of the optical frequency comb using a reference to Rubidium clock is 194 369 489 388 579 Hz， with the relative extended uncertainty of 5.8 × 10-11 （k = 2）， a calibrated value of -694 Hz， and an average stability of 4.5 × 10-12 @1s.The study provides a novel approach for remote calibration of optical frequency combs referenced to microwave frequency standards.
2025 Vol. 46 (2): 259-266 [Abstract] ( 24 ) HTML (1 KB)  PDF  (0 KB)  ( 1 )
267 A Power Stabilized Low Noise Frequency Synthesizer for CPT Atomic Clocks
WEN Zhiqiang, YUN Enxue, SUN Siyu, YANG Tenghui, WANG Bisong, WANG Leigang, LUO Xin, XU Jin, SUN Bingfeng, LI Wenbing, HAO Qiang
DOI: 10.3969/j.issn.1000-1158.2025.02.15
To achieve high performance coherent population trapping （CPT） atomic clocks， a high-performance frequency synthesizer with power locked is set up. The CPT atomic clocks require a microwave source to modulate laser that generates coherent two-color light which interact with the atomic system to obtain a very narrow linewidth resonance CPT signal and lock in the local frequency. Since its microwave modulation applies to the light， eliminating microwave cavity， CPT clocks can be portable or even chipped. Meanwhile， the short-term frequency stability of high-performance CPT atomic clocks have been demonstrated at the level of ~2×10-13τ-1/2（τ=1-10 000 s）. At this level， the Dick effect or intermodulation effect induced by microwave phase noise become limiting factors for the further improvement of the CPT clocks. For this， we realise a low phase 3.417 GHz noise synthesizer with power-locked. The experimental results show the absolute phase noise of the high performance power locked frequency synthesizer is -55.27 dBc/Hz， -83.85 dBc/Hz， -110.4 dBc/Hz， -129.5 dBc/Hz， -132.5 dBc/Hz at 1 Hz， 10 Hz，100 Hz，1 kHz，10 kHz. Its contribution of intermodulation effect to CPT atomic clocks is 5.0×10-14 ， which is analysed at the double low frequency modulation frequency. Residual stability is 7.99×10-15 at 1 s， which is more than one order of magnitude higher than the best CPT atomic clocks reported so far in the sampling time range τ=1~10 000 s. After power locking， the power stability is improved by five times at 10 000 s.
2025 Vol. 46 (2): 267-273 [Abstract] ( 24 ) HTML (1 KB)  PDF  (0 KB)  ( 1 )
274 Energy Center of Gravity Improvement Algorithm
ZHAO Xingyin, YANG Mingji
DOI: 10.3969/j.issn.1000-1158.2025.02.16
In order to improve the correction effect， the Fourier transform based on the real even window function is still a characteristic of the real even function. Based on the analysis of the traditional energy center of gravity method， a spectrum correction method to improve the anti-noise performance is proposed， that is， the energy center of gravity improved algorithm. Using this method， the correction accuracy of the signal with various windows is analyzed. The results show that the correction accuracy of the three spectral lines with Hanning window and Blackman window is improved by 0.002 frequency resolution and 0.017 frequency resolution respectively compared with the traditional energy center of gravity correction method. The theoretical analysis of the improved algorithm is carried out under the background of Gaussian white noise. The results show that the algorithm is accurate， reliable， anti-noise strong， unbiased estimation， and the correctness of the theoretical derivation is verified by simulation.
2025 Vol. 46 (2): 274-279 [Abstract] ( 18 ) HTML (1 KB)  PDF  (0 KB)  ( 3 )
280 Quantitative Measurement Method of Lateral Flow Immunoassay Test Paper Based on Atomic Magnetometer
JIANG Yuejin, WANG Boyu, BO Yufan, JIANG Zhiyuan, SHEN Yang1.College of Optical and Electronic Technology， China Jiliang University， Hangzhou， Zhejiang 310018， China
2.Center for Advanced Measure
DOI: 10.3969/j.issn.1000-1158.2025.02.17
Lateral flow immunoassay （LFIA） is widely used in rapid disease diagnosis due to its advantages such as easy operation，small sample dosage，and easy interpretation of results.However，how to quantitatively measure the target is still a key problem in LFIA application of.A quantitative measurement method of LFIA paper based on an atomic magnetometer was proposed using superparamagnetic nanoparticleses （MNP） as LFIA markers combined with quantum Magnetic sensing technology.The residual magnetic flux density spectrum was obtained by linear scanning of the paper to be tested by the atomic magnetometer.The logarithmic linear relationship between the magnetic moment and the concentration of the object was analyzed，and the amount of MNP contained was calculated to achieve quantitative measurement.When the distance between the magnetometer and the sample to be measured was 9mm，the linear range of Pro-Gastrin Releasing Peptide （Pro-GRP） LFIA test paper was 102~104 pg/mL with the lowest detection limit of 102 pg/mL.The number of MNP at this concentration is estimated to be 1.057×104.
2025 Vol. 46 (2): 280-286 [Abstract] ( 26 ) HTML (1 KB)  PDF  (0 KB)  ( 2 )
287 Characteristics of High and Low Order Coupled Networks in Mild Cognitive Dysfunction
ZHEN Haoyan, XU Jiasen, LI Zipeng, LI Xin
DOI: 10.3969/j.issn.1000-1158.2025.02.18
For the problem of abnormal brain functional connectivity in patients with mild cognitive impairment （MCI）， a new method of constructing high- and low-order coupled brain functional networks was proposed. Based on Pearson's correlation， the higher-order coupling network between brain regions was constructed on the basis of analyzing the functional correlation and synchronization of different brain regions， and important brain functional network feature markers such as high- and low-order coupling network characteristics， small-world properties， rich-club coefficients， and the stability of the network were analyzed. The results of the study showed that compared with the Alzheimer's disease （AD） control group， the delta frequency band of the MCI group had a significant decrease in its brain network connectivity connection strength， global efficiency and clustering coefficient （P<0.05）. Analyzing the core nodes of the functional network found that both AD and MCI groups had fewer core nodes than the normal （HC） group under each network， the core nodes of the MCI group were mainly distributed in the prefrontal lobe， and the core nodes of AD were mainly distributed in the parietal and occipital lobe， and the core node vulnerability of the AD group was significantly higher （P<0.05） compared to the HC group in the beta and delta frequency bands， whereas the core node vulnerability of the MCI group was significantly higher （P<0.05） than the normal （HC） group in the beta and theta bands， the core node susceptibility of MCI group was significantly larger than that of HC group （P<0.05）. The above results suggest that the high- and low-order coupled network features can effectively express the functional state of the brain in mild cognitive impairment， which will be helpful for the early diagnosis of mild cognitive impairment.
2025 Vol. 46 (2): 287-297 [Abstract] ( 19 ) HTML (1 KB)  PDF  (0 KB)  ( 2 )
298 Evaluation of the Effect of Different Conditions on Single Cell Preparation of Tumorsphere
YANG Lulu, ZHANG Yongzhuo, GONG Xiaoyun, XUE Zhichao, SHENTU Xuping
DOI: 10.3969/j.issn.1000-1158.2025.02.19
The choice of single-cell isolation and preparation techniques can significantly impact cell status and physiological functions， ultimately leading to biases in related studies. This study employs a tumor spheroid model with strong cultivation consistency and utilizes four separation methods， including water bath， metal bath， mechanical， and optimized water bath methods， along with three types of dissociation enzymes—collagenase， trypsin， and a human tumor gentle enzyme kit—to isolate multiple single-cell sample groups. Key indicators such as single-cell quantity， viability， metabolic products， and drug pathways were investigated. The results indicate that the optimized water bath method yields the highest single-cell viability at （90.27±8.56）%， while trypsin demonstrates the best performance among the enzymes， with a single-cell viability of （93.20±3.84）% and the highest cell yield. Collagenase-prepared single cells exhibit the strongest clonal proliferation ability and express fewer metabolic changes compared to the tumor spheroid， closely resembling the true state of the tumor spheroid. Trypsin-prepared single cells show downregulation of metabolites involved in methionine metabolism， reducing the sensitivity of cancer cells to drugs and affecting the assessment of drug efficacy.
2025 Vol. 46 (2): 298-308 [Abstract] ( 9 ) HTML (1 KB)  PDF  (0 KB)  ( 2 )
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