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| Characterizing on Genetically Modified T25 Maize Line Matrix Reference Material |
| DONG Lian-hua1,LI Liang1,ZHOU Yun-long2,CAO Ying-Long3,SHEN Ping2,LU Chang-ming3,WANG Jing1,LIU Zhang-lan1 |
1. National Institute of Metrology, Beijing 100013, China ;
2. Science and Technology Development Center, Ministry of Agriculture, Beijing 100122, China;
3. Oil Crops Research Institute of Chinese Academy of Agricultural Science, Wuhan, Hubei 430062, China |
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Abstract To validate the key parameters of the real time PCR method for GM maize T25, 7 different laboratories are chosen to assign the reference values of three different GM content matrix reference materials by using the same method.Although different types of instruments are used by different labs, the standard curve generated by each lab is comparable which indicated that this method shows a good reproductablity.After statistically analyzing the data gained from seven labs, the total means are assigned to be the reference values.The reference values and expanded uncertainties (k=2) for three matrix GM reference material are 1.17%±0.22%,2.17%±0.38% and 9.81%±2.30%.
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2014, Vol. 35 
