Abstract For the needs of medical excimer laser light source traceability, the excimer laser treatment machine online verification system was developed which consisting the simulated eye movement systems, simulation of human cornea and ultraviolet laser energy test device. Simulated eye movement system consists of PLC and two-dimensional motion platform, the human cornea was simulated by Polymethy methacrylate calibration plate, and an ultraviolet laser power meter was used to measure ultraviolet laser energy. The test results show that the system basically achieved the predefined detection capabilities and positioning accuracy.