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| Techniques on accurate qualitative characterization of therapeutic oligonucleotides |
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Abstract Therapeutic oligonucleotide with chemical groups modification has been used for the treatment of specific diseases with significant superiorities. The sequence of therapeutic oligonucleotide is designable, for different loci of interest can be targeted by hybridization. However, the sequence of the oligonucleotides can cause risk when being delivered into target regions because of off-target effects or unknown side-effect. And the length of the synthesized products can also be another problem. The modification of therapeutic oligonucleotides is able to prevent its degradation and prolongates the effective drug duration. The solid phase process of the therapeutic oligonucleotides could produce structural analogues of the parent oligonucleotides, thus introducing product impurities. The accurate sequencing and structure characterization of therapeutic oligonucleotides will provide key information for their synthesized product and clinical research. The sequencing techniques and separation techniques of therapeutic oligonucleotides are aim to offer chemical information of the detailed structure, including length, sequence, and impurities, thus the quality control of producing and drug safety assessment can be benefited from the introductions of these techniques.
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Received: 25 April 2024
Published: 19 March 2025
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2025, Vol. 46 
