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| Research Advances of Reference Materials in Absolute Quantification of Microbiome |
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Abstract Microbiome research based on amplicon and metagenomic sequencing can help to analysis microbial community composition and function, and is an important tool for the study of microbial structure ecology and interaction mechanism. In order to represent the host-microbiome interactions, microbiome sequencing is gradually changing from relative abundance to absolute quantitative analysis of community composition. Reference material can be used as a standardized factor to accurately quantify the total load of each microorganism in the community. The reference materials for absolute quantification of microbiome, including microbial consortia, microbial genome, internal standard strains and spike-in sequences, and their applications were reviewed in this paper, the development trend of reference materials in quality control and standardization of microbiome is also proposed, so as to call for further expanding the application range of reference materials for absolute quantification for microbiome in microbial ecology.
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Received: 13 March 2024
Published: 19 March 2025
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Corresponding Authors:
lianhua dong
E-mail: lianhuadong@126.com
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WEN Zhiqiang, YUN Enxue, SUN Siyu, YANG Tenghui, WANG Bisong, WANG Leigang, LUO Xin, XU Jin, SUN Bingfeng, LI Wenbing, HAO Qiang. A Power Stabilized Low Noise Frequency Synthesizer for CPT Atomic Clocks[J]. Acta Metrologica Sinica, 2025, 46(2): 267-273. |
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2025, Vol. 46 
