基于Lambert-Beer定律的吸光度监测微容量液体体积测量系统及方法研究

doi:10.3969/j.issn.1000-1158.2025.02.07

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Abstract To overcome the shortcoming of hydro-weighing method，which has high environmental requirements， liquid evaporation affects accuracy and is difficult to realize field detection， a micro liquid volume measurement method was studied based on Lambert-Beer. To improve measurement repeatability， a measurement system with absorbance monitoring optical path was designed to monitor interference factors such as light source fluctuations，and a correction coefficient was proposed. A measurement scheme was designed，and corresponding mathematical analytical model was proposed to calculate micro liquid volume. A practical measurement system was built and experiments were carried out. The liquid mixing experiment verified that the measurement efficiency was improved. The measurement experiments carried out on the volume detection point of 100 μL verified that the absorbance monitoring optical path can improve the repeatability from 2.02% to 0.76%. The final measurement results meet the requirements of JJG 646-2006， which verifies the effectiveness of the measurement system.

Key words： volume measurement micro liquid volume Lambert-Beer law absorbance monitoring

Received: 08 October 2023 Published: 04 March 2025

PACS:

TB938.3

Corresponding Authors: Jintao Wang E-mail: 16142751@qq.com

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	CHANG Xu
	WANG Jintao
	LIU Xiang
	ZHANG Jingyue
	TONG Lin
	GUO Ligong
	XU Changhong
	SHI Wencai
	XU Haoming

Cite this article:

CHANG Xu,WANG Jintao,LIU Xiang, et al. Research on Absorbance Monitoring Micro Liquid Volume Measurement System and Method Based on Lambert-Beer Law[J]. Acta Metrologica Sinica, 2025, 46(2): 198-203.

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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2025.02.07 OR http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2025/V46/I2/198

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