基于UPLC-DAD-CAD双检测器串联的DON纯度测量方法对比研究

doi:10.3969/j.issn.1000-1158.2024.07.19

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Abstract An ultra-high performance liquid chromatography with diode array detector coupled to charged aerosol detector (UPLC-DAD/CAD) method was established for comparative study on deoxynivalenol (DON) purity analysis.The optimized chromatographic condition was isocratic elution with 6% methanol for 20 minutes.There were obvious differences in the types and amounts of impurities detected by the two detectors.17 impurities were identified by DAD with impurity 15 accounting for the highest proportion (0.847%), following by impurity 17 (0.0812%) and impurities 2, 5 and 8 (above 0.01%).9 impurities were identified by CAD with impurity 17 accounting for the highest proportion (0.4060%), following by impurity 15 (0.3499%) and impurities 10, 11 and 12 (above 0.1%).The purity obtained by DAD detection method was 98.92%, significantly higher than that obtained by CAD detection method (98.61, p<0.001).And the relative standard deviation of DAD method (0.00670%) was also lower than that of the CAD method (0.0461%).The measurement uncertainty of purity by the DAD method was 0.463%, mainly introduced by response differences of the impurities; while it was 0.0503% for the CAD method, which was mainly introduced by measurement repeatability.The results may be helpful for the chromatographic purity analysis of DON.

Key words： biometrology;deoxynivalenol chromatographic purity analysis DAD CAD uncertainty

Received: 26 September 2023 Published: 04 July 2024

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TB99

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	WANG Songshan
	LEI Da
	JIA Ruihui
	LI Li
	ZHOU Minghui
	WANG Songxue

Cite this article:

WANG Songshan,LEI Da,JIA Ruihui, et al. Comparative Study on DON Purity Analysis Based on UPLC with DAD Coupled to CAD[J]. Acta Metrologica Sinica, 2024, 45(7): 1081-1088.

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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2024.07.19 OR http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2024/V45/I7/1081

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