数字PCR法在食品检测标准物质研制中的应用

doi:10.3969/j.issn.1000-1158.2023.03.21

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Abstract Food detection reference material is a measuring instrument to ensure traceability, mutual recognition and accuracy of food detection value, and its development needs accurate determination methods. Digital PCR is a sensitive, accurate and matrix-resistant method for absolute quantification of nucleic acid, which is widely applied in many fields such as medical diagnosis, genetically modified organism detection and animal disease detection. Digital PCR method and its research progress in the detection of genetically modified foods, animal and plant origin analysis and the development of food-borne pathogenic bacteria detection reference materials is reviewed to provide reference for the development of food detection nucleic acid reference materials.

Key words： metrology food detection reference material digital PCR

Received: 18 June 2022 Published: 08 March 2023

PACS:

TB99

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	Articles by authors
	FAN Hong-bo
	CAI Yong-hong
	LI Yue-yue
	HUANG Man-ying
	ZHANG Li-ling
	MA Ting-ting
	HU Song-qing
	HU Liang-yong

Cite this article:

FAN Hong-bo,CAI Yong-hong,LI Yue-yue, et al. Application of Digital PCR in Development of Food Detection Reference Materials[J]. Acta Metrologica Sinica, 2023, 44(3): 472-478.

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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2023.03.21 OR http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2023/V44/I3/472

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