耐除草剂转基因大豆SHZD32-1数字PCR绝对定量方法的建立

doi:10.3969/j.issn.1000-1158.2023.03.14

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Abstract Based on the digital PCR technology for absolute quantification of DNA single molecule, a droplet digital PCR (ddPCR) dual (transformant-specific gene and soybean internal standard gene) quantitative method for the transformant was established. The main contents include:optimize the double ddPCR primer-probe concentration and annealing temperature, examine the specificity, determine the linear kinetic range and critical parameters such as the limit of detection (3copies/μL) and the limit of quantification (15copies/μL). The genetically modified soybean SHZD32-1 has obtained the production and application safety certificate "Nongjianzhengzi (2019)" No. 293. Its ddPCR quantitative detection method, as an absolute quantitative measurement method, is simple, efficient and accurate, and will provide technical support for the calibration of reference materials, quantitative detection and standardization of the establishment of digital PCR methods in the field of biosafety.

Key words： metrology genetically modified detection;digital PCR absolute quantitative method genetically modified soybean reference material

Received: 21 February 2022 Published: 08 March 2023

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TB99

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	Articles by authors
	ZHENG Zi-fan
	WANG Hao-qian
	GAO Jia-qi
	CHEN Shuo
	LIU Fang-fang
	ZHANG Xiu-jie
	LI Liang

Cite this article:

ZHENG Zi-fan,WANG Hao-qian,GAO Jia-qi, et al. Development of Digital PCR Absolute Quantification Method for Herbicide-tolerant Genetically Modified Soybean SHZD32-1[J]. Acta Metrologica Sinica, 2023, 44(3): 415-421.

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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2023.03.14 OR http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2023/V44/I3/415

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