一种模拟游离DNA阳性质控品的制备方法及表征

doi:10.3969/j.issn.1000-1158.2023.03.07

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Abstract By optimizing a new type of enzymatic hydrolysis system with unknown mechanism (reaction system, RS), which directly acts on tumor cell lines SW1573,SNU-C2B and NCI-H1975, with KRAS G12C,KRAS G12D,EGFR L858R and EGFR T790M mutations, by optimizing the action time and cell number of RS system, a kind of positive quality control substance which can highly mimic human plasma free DNA (cfDNA) can be prepared by cleavage of cell genomic DNA. The length of simulated cfDNA fragments detected by chip electrophoresis is (166±16)BP, which is the same as that of normal human plasma cfDNA. Microdrop digital PCR detection show that the cfDNA fragments prepared by RS and gDNA have the same mutation abundance at the target mutation site. The results show that the DNA fragment prepared by RS system can be used as a positive quality control substance of cfDNA.

Key words： metrology cell free DNA positive quality control substance simulated cfDNA mutation abundance ddPCR

Received: 17 March 2021 Published: 08 March 2023

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TB99

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	Articles by authors
	FANG Yan
	WANG Xia
	DONG Lian-hua
	YANG Jing-ya

Cite this article:

FANG Yan,WANG Xia,DONG Lian-hua, et al. Preparation and Characterization of a Simulated Cell Free DNA Positive Quality Control Material[J]. Acta Metrologica Sinica, 2023, 44(3): 361-367.

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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2023.03.07 OR http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2023/V44/I3/361

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