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| Cell Growth and Drug Effect Differences Induced by Inoculum Number Variations and Comparison of Cell Counting Methods |
| XUE Zhi-chao1,ZENG Jia-ming1,LI Yong-shu2,ZHAO Jia-wei1,ZHAO Yang1,DAI Xin-hua1,GONG Xiao-yun1 |
1. Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, China
2. Shenzhen Institute for Technology Innovation, National Institute of Metrology, Shenzhen,Guangdong 518055, China |
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Abstract Hela cells were used to investigate the changes in cell growth rate and the influence of drug effect induced by the cell number differences at the cell seeding step. The experimental results indicated that the larger the initial number of cells, the higher the density after adhesion, the faster the growth rate, and the lower the inhibitory effect of drugs on its growth. Therefore, the accuracy and efficiency of cell counting are of great importance for in vitro studies. Three commonly used cell counting methods were compared including the cell counting plate method, the cell counting apparatus method, and the flow cytometry method. Their linear regression R2 were 0.9835, 0.9997, and 0.9960 respectively. Under the same condition, the cell counting apparatus method held the highest accuracy. In addition, indirect methods such as crystal violet staining, basal area photography, protein content estimation, and protein reference GAPDH measurement were also evaluated in to provide data reference for cell number measuring in biological experiments.
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Received: 04 July 2022
Published: 08 March 2023
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2023, Vol. 44 
