1. Shandong Institute for Food and Drug Control, Jinan, Shandong 250100, China
2. College of Chemical and Pharmaceutical Engineering,Shandong First Medical University, Taian, Shandong 271016, China
Abstract The formula food for special medical use is the only source of nutrition for some people, and the accurate quantification of biotin is of great significance. The microbiological method specified in GB 5009.259—2016 was used to determine the content of biotin. The standard uncertainty and expanded uncertainty of biotin were evaluated. The results showed that the content of biotin was (27.16±1.15)μg/100g, p=95%, k=2. The contribution of each component to the uncertainty was analyzed. The results showed that the experimental repeatability was the main influencing factor and the key control point for the determination of biotin by microbiological method.
MENG Jing,WU Yu-jian,ZHONG Li-xia, et al. Uncertainty Evaluation for Microbiological Determination of Biotin in Food for Special Medical Use[J]. Acta Metrologica Sinica, 2021, 42(10): 1404-1408.
［1］León-Del-Río A. Biotin in metabolism, gene expression, and human disease［J］. Journal of Inherited Metabolic Disease, 2019, 42(4): 647-654.
［2］Ali A E, Hamed M. Effect of Biotin on Patients with Multiple Sclerosis in a Sample of Egyptian Patients［J］. Multiple Sclerosis and Related Disorders, 2018, 26: 241-242.
［3］陶大利, 白鹤, 李琴. SPE净化-同位素稀释质谱法测定乳与乳制品中生物素的含量［J］. 现代食品, 2018,(21): 103-106.
Tao D L, Bai H, Li Q. Determination of Biotin in Milk and Dairy products by SPE Purification-Isotope Dilution Mass Spectrometry［J］. Modern Food, 2018,(21): 103-106.
［4］刘进玺, 钟红舰, 董小海. 超高效液相色谱-质谱联用测定维生素预混合饲料中生物素含量［J］. 分析试验室, 2010, 29(7): 62-64.
Liu J X, Zhong H J, Dong X H. Determination of Biotin in premix feed with UPLC/MS/MS［J］. Chinese Journal of Analysis Laboratory, 2010, 29(7): 62-64.
［5］王凤玲, 刘爱国, 孙佳佳, 等. 高效液相色谱-质谱法同时测定婴幼儿配方奶粉中叶酸、VB_(12)和生物素［J］. 食品科学, 2013, 34(22): 269-272.
Wang F L, Liu A G, Sun J J, et al. Simultaneous Determination of Folic Acid, Vitamin B12 and Biotin in Infant Formula by HPLC-MS-MS［J］. Food Science, 2013, 34(22): 269-272.
［6］裘丞军, 任玉琴. 高效液相色谱法测定饲料添加剂D-生物素的含量［J］. 中国饲料, 2018,(19): 80-84.
Qiu C J, Ren Y Q. Determination of D-biotin in feed additives by HPLC［J］. China Feed, 2018,(19): 80-84.
［7］佟晓芳, 张永胜, 陶大利, 等. 免疫亲和柱净化-高效液相色谱法测定乳粉中的生物素含量［J］. 现代食品科技, 2013, 29(3): 660-663.
Tong X F, Zhang Y S, Tao D L, et al. Determination of Biotin Content in Milk Powder by Immunoaffinity Column Purification-HPLC［J］. Modern Food Science and Technology, 2013, 29(3): 660-663+557.
［8］陈金东, 韩素平, 李蔚. 营养强化剂类保健食品中生物素含量的测定［J］. 中国卫生检验杂志, 2011, 21(9): 2160-2161.
Chen J D, Han S P, Li W. Determination of biotin in health-care food of nutrition enhancers［J］. Chinese Journal of Health Laboratory Technology, 2011, 21(9): 2160-2161.
［9］杜碧莹, 刘丽芳. 注射用水溶性维生素中生物素含量测定方法改进［J］. 临床合理用药杂志, 2010, 3(2): 11-13.
Du B Y, Liu L F. Improve content determination method of biotin in water-soluble vitamins for injection［J］. Chinese Journal of Clinical Rational Drug Use, 2010, 3(2): 11-13.
［10］郭法利, 施杨. 食品中生物素测定方法的研究进展［J］. 食品工业, 2019, 40(3): 219-222.
Guo F L, Shi Y. Recent Progress of Determination Methods on Biotin in Foods［J］. The Food Industry, 2019, 40(3): 219-222.
［11］李秋顺, 郑晖, 孟庆军, 等. 生物素的测定方法及应用进展［J］. 食品工业, 2012, 33(5): 103-107.
Li Q S, Zheng H, Meng Q J, et al. Progress of Determination Methods and Applications of Biotin［J］. The Food Industry, 2012, 33(5): 103-107.
［12］王志伟. 婴幼儿乳粉中生物素含量的测定研究［J］. 包装与食品机械, 2013, 31(3): 66-68.
Wang Z W. Research on Determination of Biotin in Infant Milk Powder［J］. Packaging and Food Machinery, 2013, 31(3): 66-68.
［13］秦磊磊, 陈绪华, 刘健, 等. 微生物法测定婴幼儿奶粉中游离生物素条件优化［J］. 现代农业科技, 2013,(15): 296-297.
Qin L L, Chen X H, Liu J, et al. Optimization of Determination Conditions of Free Biotin in Infant Milk Powder by Microbiological Method［J］. Modern Agricultural Science and Technology, 2013,(15): 296-297.
［14］GB5009. 259-2016 食品安全国家标准 食品中生物素的测定［S］. 北京: 中国标准出版社, 2016.
［15］贾未鸣, 高旭锋, 凌凤香, 等. 微晶蜡含油量不确定性评估［J］. 计量学报, 2020, 41(7):886-890.
Jia W M, Gao X F, Ling F X, et al. Uncertainty Evaluation for the Oil Content of Microcrystalline Wax［J］. Acta Metrologica Sinica, 2020, 41(7): 886-890.
［16］张川, 罗嫣, 于涛, 等. 中国一级标准海水不确定度分析研究［J］. 计量学报, 2020, 41(8): 1027-1032.
Zhang C, Luo Y, Yu T, et al. Estimating the Uncertainty of China Primary Standard Seawater［J］. Acta Metrologica Sinica, 2020, 41(8): 1027-1032.
2021, Vol. 42 
