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Rapid and Quantitative Detection of Viable Staphylococcus aureus in Milk Powder Based on Flow Cytometry Technology |
WANG Bin1,2,SUI Zhi-wei2,LIU Si-yuan2,WANG Jing2,FU Bo-qiang2,WANG Yi1 |
1. College of Life Science, Jilin Agricultural University, Changchun,Jilin 130118, China
2. Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029, China |
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Abstract A flow cytometry (FCM)-based method for rapid and quantitative detection of viable Staphylococcus aureus in milk powder was developed. Staphylococcus aureus was labelled with Fluorescein isothiocyanate (FITC) by reacting with the anti-Staphylococcus aureus polyclonal antibody (FITC), then dead and viable bacteria were distinguished by membrane-impermeable dyes Propidium Iodid (PI). The FCM-based method was established and applied to the quantitative detection for Staphylococcus aureus in milk powder samples. The results showed that the FCM-based method exhibited high specificity towards Staphylococcus aureus. When the concentration of Staphylococcus aureus in artificially contaminated milk powder samples ranged from 2.72×103~2.80×108CFU/mL, the FCM-based method and plate-based method yielded the same result, and the detection limit of the method is 2.72×103CFU/mL, and total time is 35min. The method is rapid and efficient, so it has a great application prospect in rapid screening and monitoring of foodborne pathogens.
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Received: 22 February 2019
Published: 18 February 2021
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Fund:National Key R&D Program of China;National Key R&D Program of China |
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