Study on Measurement System for Bacteriophage ΦX174 Reference Material
LIU Si-zhang1,2,SUI Zhi-wei2,ZHANG Tao3,WANG Jing2,LIU Xiao-xia2, WANG Yi1,ZHANG Mei-ping1
1.College of Life Science, Jilin Agricultural University, Changchun, Jilin 130118, China
2.National Institute of Metrology, Beijing 100029, China
3.Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei 430071, China
Abstract The double-layer agar counting method of phage ΦX174 in international standard (ISO 16604) was adopted as the calibration method of phage ΦX174 standard substance, and the method was validated by the method and verified by synergistic experiment and the uncertainty analysis. The results showed that the repeatability and reproducibility of the method were good, the relative standard deviation of indoor repeatability was 10.18%, and the relative standard deviation was 14.58%. According to the statistical analysis, the relative expansion uncertainty of the double agar counting method was 12.44%, which was suitable for the determination of the standard substance of phage ΦX174, which was very important for the evaluation of protective performance of personnel protective equipment.
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2019, Vol. 40 
