Acta Metrologica Sinica  2024, Vol. 45 Issue (2): 294-299    DOI: 10.3969/j.issn.1000-1158.2024.02.21
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Research of a Reference Material of Inactivated Influenza A (H1N1) Virus
XU Li1,YUE Lihuan2,YANG Xue1,WANG Zhaokun1,LI Lanying1,WEN Yanli1,TAO Qing1,HU Yihong2,LIU Gang1
1.Key Laboratory of Bioanalysis and Metrology for State Market Regulation, Shanghai Institute of Measurement
and Testing Technology, Shanghai 201203, China
2. Institutional Center for Shared Technologies and Facilities, Pathogen Discovery and Big Data Platform, Shanghai Institute of Immunity and Infection, Chinese Academy of Sciences, Shanghai 200031, China
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Abstract  A reference material (RM) of inactivated influenza A (H1N1) virus was developed. The RM was made of artificially cultured natural virus as raw materials. After chemical inactivation and verification of the inactivation effect, the copy number concentration was determined by digital PCR (dPCR) in different laboratories. The homogeneity, stability and uncertainty of the RM were evaluated in detail. As the results demonstrated, the RM can be stably stored for more than 5 months under -80~-70℃. The final certified value of reference materials was (1384.5±249.3) copies/μL (k=2). The RM can be used for the metrological traceability and quality control in the whole process of influenza A virus H1N1 detection and help to improve the accuracy and reliability of related nucleic acid test results.
Key wordsbiometrology      influenza A virus, H1N1, inactivated virus, reference material, digital PCR     
Received: 04 August 2023      Published: 21 February 2024
PACS:  TB99  
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XU Li
YUE Lihuan
YANG Xue
WANG Zhaokun
LI Lanying
WEN Yanli
TAO Qing
HU Yihong
LIU Gang
Cite this article:   
XU Li,YUE Lihuan,YANG Xue, et al. Research of a Reference Material of Inactivated Influenza A (H1N1) Virus[J]. Acta Metrologica Sinica, 2024, 45(2): 294-299.
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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2024.02.21     OR     http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2024/V45/I2/294
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