Abstract After the heart type fatty acid binding protein (H-FABP) is hydrolyzed by acid hydrolysis, the amount of four derivatives of aspartic acid, glutamic acid, histidine and arginine can be determined, and then the content of pure H-FABP can be calculated. During the determination process, the national standard substance of amino acid mixed solution was used as the standard, γ-aminobutyric acid was used as the internal standard, and treated with pre column derivatizer. Comparing the results of this method with those of isotope dilution mass spectrometry for the determination of H-FABP content can verify the accuracy of the established method. The experiment showed that under the optimal conditions, the H-FABP content in the established pre column derivatization ultra high performance liquid chromatography (UPLC) method was determined to be 0.1113mg/mL. The relative standard deviation was 2.03%, the expanded uncertainty was 0.0027mg/mL (k=2), and the detection limit and quantification limit were 1.16×10-3mg/mL and 3.86×10-3mg/mL, respectively. In addition, this method has a low cost and clear traceability chain, which may serve as a supplementary validation method for the calibration of pure H-FABP reference materials.
CUI Hong-en,FENG Su,YE Ren-yuan, et al. Determination of the Mass Fraction of Pure Heart-type Fatty Acid-binding Protein by Pre-column Derivatization UPLC[J]. Acta Metrologica Sinica, 2023, 44(7): 1141-1146.
Wang Y Y, Gao D S. The progress in applications of heart-type fatty acid binding protein in the diagnosis of acute myocardial infarction ［J］. Anhui Medical and Pharmaceutical Journal,2017,21(11):1968-1971.
［4］
Apple F S, Sandoval Y, Jaffe A S, et al. Cardiac Troponin Assays: Guide to Understanding Analytical Characteristics and Their Impact on Clinical Care［J］. Clinical Chemistry: Journal of the American Association for Clinical Chemists,2017,63(1):73-81.
Sandhu A T, Heidenreich P A, Bhattacharya J, et al. Cardiovascular Testing and Clinical Outcomes in Emergency Department Patients With Chest Pain［J］. JAMA internal medicine,2017,177(8):1175-1182.
［9］
ISO 17511:2020, In vitro diagnostic medical devices—Measurement of quantities in biological samples—Metrological traceability of values assigned to calibrators and control materials ［S］.
Wang P Y, Zhou Y, Huang P, et al. Determination of Ampicillin and Amoxicillin Residues in Aquatic Product Using Reversed-phase High Performance Liquid Chromatography-fluorescence Detection with Pre-column Derivatization［J］.Acta Metrologic Sinica,2012,33(4): 377-380.
［17］
JJG 196—2006 常用玻璃量器检定规程［S］. 2006.
Li L, Jia Z, Zhou J, et al. Uncertainty evaluation of tryptophan in fishmeal by high performance liquid chromatography method ［J］. Journal of Food Safety & Quality, 2020,11(9):2714-2721.
Ono T. Studies of the FABP family: A retrospective［J］. Molecular and Cellular Biochemistry: An International Journal for Chemical Biology,2005,277(1/2):1-6.
Xiang X, Yang L, Guo Y X, et al. Diagnosis Accuracy of Heart- type Fatty Acid- Binding Protein in Acute Myocardial Infarction: A Systematic Review and Meta- Analysis［J］. Xinjiang Medical Journal,2021,51(04):409-414.
［5］
Mcmahon C G, Lamont J V, Curtin E, et al. Diagnostic accuracy of heart-type fatty acid-binding protein for the early diagnosis of acute myocardial infarction［J］. The American journal of emergency medicine,2012,30(2):267-274.
Zhao Y L, Li W M. Research Progress of Heart-type Fatty Acid-binding Protein in Cardiovascular Diseases ［J］. Adv Cardiovasc Dis,2018,39(4):636-639.
Li J L, Wu L Q, Jin Y X, et al. Determination of the Mass Fraction of Pure Human Serum Albumin by HPLC-IDMS［J］. Acta Metrologic Sinica,2016,37(3): 328-332.
Li J L, Wu L Q, Liu W L, et al. Determination on mass fraction of pure human transferrin through high performance liquid chromatography-isotope dilution mass spectrometry ［J］. China Measurement & Test, 2015, 41(5): 58-62.
Ding S, Huang H, Hu Y. Research progress on amino acids analysis ［J］. Chinese Journal of Bioprocess Engineering, 2018, 16(3):12-21.
He J J, Zhang X F, Qian L L, et al. Research progress in trace amino acid analysis methods ［J］. Journal of Analytical Science,2019,35(4):507-513.
Song D W, Dong X J, Xu B, et al. Measurement and International Comparison for C-Reactive Protein by Isotope Dilution Mass Spectrometry［J］. Journal of Chinese Mass Spectrometry Society,2014,35(5):462-466.
2023, Vol. 44 
