1.National Institute of Metrology,Beijing 100029,China
2. College of Food Science and Technology,Agricultural University of Hebei, Baoding,Hebei 071001, China
3. Hebei Key Laboratory of Analysis and Control for Zoonotic Pathogenic Microorganism, Agricultural University of Hebei, Baoding,Hebei 071001,China
4. School of Life Science and Technology, Wuhan Polytechnic University, Wuhan, Hubei 430023, China
5. College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China
Abstract Microbial reference materials (RMs) in dairy products are important tool for developing microbiological measurement techniques, establishing microbial detection standards, and improving the quality supervision system of milk and dairy products, which have broad application prospects. The main characteristics of the microbial RMs, and the main preparation technology that could help with the microbial activity, as well as the measurement techniques of the quantity value and nominal property are introduced. Furthermore, an overview on the development and application of the microbial RMs in dairy products are given. Based on the analysis of the main problems existing in this field, the demand and trend of the RMs are prospected, which can provide more meaningful reference for the subsequent research of microbial RMs.
LIU Si-yuan,BAI Zhao-ying,WANG Zi-quan, et al. Research Status and Trend of MicrobialReference Materials in Dairy Products[J]. Acta Metrologica Sinica, 2023, 44(3): 457-463.
McKinnon K M. Flow Cytometry: An Overview［J］. Curr Protoc Immunol, 2018, 120: 5.1.1-5.1.11.
Liu X Y, Zhang Y, Chen L J, et al. Research on testing microorganisms in raw milk［J］. China Food Additives, 2013(2):169-174.
Zhang X G, Huang T, Zhang W, et al. Development of a Certified Reference Material for Lyophilized Vitamin K1［J］. Acta Metrologica Sinica, 2015,36(3):333-336.
［5］
Miyamoto-Shinohara Y, Sukenobe J, Imaizumi T, et al. Survival curves for microbial species stored by freeze-drying［J］. Cryobiology, 2006, 52(1):27-32.
［6］
Fonseca F, Cenard S, Passot S. Freeze-drying of lactic acid bacteria［J］. Methods Mol Biol, 2015, 1257:477-488.
Bi J F, Feng S H, Jing X, et al. Current Status and Development Trend of Freeze Drying Technology and Industry［J］. Journal of Nuclear Agricultural Sciences, 2022,36(2):414-421.
Givan A L. Flow cytometry: an introduction［J］. Methods Mol Biol, 2011, 699: 1-29.
［2］
Saraiva L, Wang L, Kammel M, et al. Comparison of Volumetric and Bead-Based Counting of CD34 Cells by Single-Platform Flow Cytometry［J］. Cytometry B Clin Cytom, 2019,96(6):508-513.
［8］
Jiao R, Gao J, Zhang X, et al. Short communication: Effects of vacuum freeze-drying on inactivation of Cronobacter sakazakii ATCC29544 in liquid media with different initial inoculum levels［J］. J Dairy Sci, 2017, 100(3):1674-1678.
Wolfe J, Bryant G. Freezing, drying, and/or vitrification of membrane-solute-water systems［J］. Cryobiology, 1999, 39(2):103-129.
Xue F, Wang F S. The Ｒesearch Progress in Lyoprotectants of Protein and Its Protection Mechanism［J］.Chinese Pharmaceutical Journal, 2018,53(10):765-770.
Jin J H, Sun X M, Chen W H. Preliminary Study of Microorganism Variation In Vacuum Freeze Drying Process［J］. Chinese Wild Plant Resources, 2019,38(4):48-52.
Wang J, Zhu Z Z, Zhang X H. Spray freeze drying technology and its application in inhalation preparations［J］. Chinese Journal of Pharmaceuticals, 2018,49(8):1083-1090.
Yang J, Peng R L,Wang P, et al. Recent Development of Spray Freeze Drying Technology and Equipment［J］.Vacuum, 2022,59(2):72-80.
Colombo M, de Oliveira A E, de Carvalho A F, et al. Development of an alternative culture medium for the selective enumeration of Lactobacillus casei in fermented milk［J］. Food Microbiol, 2014, 39:89-95.
［25］
Liu S, Sui Z, Lin J, et al. Rapid detection of single viable E.coli O157:H7 cells in milk by flow cytometry［J］. Journal of Food Safety, 2019, 39(4):1-2.
Sundaramurthi P, Suryanarayanan R. Influence of crystallizing and non-crystallizing cosolutes on trehalose crystallization during freeze-drying［J］. Pharm Res, 2010, 27(11):2384-2393.
Chen M F, Fu B Q, Lin J, et al. Development of Quantitative Reference Material of Enterobacter Cloacae［J］.Acta Metrologica Sinica, 2017,38(5):527-531.
Yan B H, Han Y J, Yang H, et al. Application of Spray Drying Technology in Industry［J］. Mechanical and Electrical Information, 2022(12):86-88.
Kuang M J, Jiao T T, Sun Y, et al. Research progress on Drying Technology of Purple Sweet Potato Powder［J］. Grain Processing, 2021,46(5):57-59.
Wang Z, Xu Q, Liu S, et al. Rapid and multiplexed quantification of Salmonella, E.coli O157:H7, and Shigella flexneri in ground beef using flow cytometry［J］. Talanta, 2022, 238(1): 123005.
［30］
BIPM.Consultative Committee for Amount of Substance-Metrology in Chemistry and Biology Working Group on Cellular Analysis: Strategy 2021—2030［EB/OL］. https://www.bipm.org/en/strategy
Dong L H, Sui Z W, Wang J, et al. Accurate quantification of Plasmid DNA copy concentration by digital PCR［J］. Acta Metrologica Sinica, 2017,38(2):247-251.
Zhang S, He X W, Liu X Y, et al. Rapid Detection of Listeria monocytogenes Using a Fluorescence Immunochromatographic Assay Combined with Immunomagnetic Bead Separation［J］. Modern Food Science and Technology, 2014,30(11):229-234.
［38］
VeldPHI, Notermans S. Use of Reference Materials (Spray-Dried Milk Artificially Contaminated with Salmonella typhimurium) to Validate Detection Methods for Salmonella［J］. J Food Prot, 1992, 55(11):855-858.
［39］
Da Silva S M, Vang L K, Olson N D, et al. Evaluation of microbial qPCR workflows using engineered Saccharomyces cerevisiae［J］. Biomolecular detection and quantification, 2016,7: 27-33.
Chen A L, Hu Y X, Zhang Y J, et al. The Protective Effect of Combination Cryoprotectants on Viable Bacteria in Fecal Suspension［J］. Genomics and Applied Biology, 2021,40(4):3776-3786.
Xue L, Lin J, Sui Z W, et al. Preparation of Reference Material for Quantitative Detection of E.coli［J］.Acta Metrologica Sinica, 2015,36(6):652-656.
Liu S Y, Wang Z Q, Zhen X X, et al. Development of reference materials of Escherichia coli for productivity ratio evaluation of microbiological media［J］.China Measurement & Test, 2020,46(10):48-53.
［29］
ISO 17511—2020 In vitro diagnostic medical devices-Requirements for establishing metrological traceability of values assigned to calibrators, trueness control materials and human samples［S］.
［35］
Ma J, Prince A, Aagaard K M. Use of whole genome shotgun metagenomics: a practical guide for the microbiome-minded physician scientist［J］. Semin Reprod Med, 2014, 32(1):5-13.
Zhai H R, Luo H P, Li J Y, et al. Preparation of Escherichia coli reference materials for food detection［J］. Journal of Food Safety & Quality, 2021,12(17):6763-6769.
Li Y, Luo C, Li L Y, et al. Homogeneity and Stability Study on Standard Reference Materials of Staphylococcus aureus in Milk Powder［J］. Chemical Reagents, 2019,41(2):177-181.
Liu S Z, Sui Z W, Zhang T, et al. Study on Measurement System for Bacteriophage ΦX174 Reference Material［J］. Acta Metrologica Sinica,2019, 40(3): 511-516.
Wang B, Sui Z W, Liu S Y, et al. Rapid and Quantitative Detection of Viable Staphylococcus aureus in milk powder based on flow cytometry technology［J］. Acta Metrologica Sinica, 2021,42(2):250-258.
Meng L Y, Niu Q Y, Lian L X, et al. Identification of Salmonella and Staphylococcus aureus Using 16S rDNA Sequencing, MALDI-TOF-MS and VITEK System［J］. Journal of Chinese Institute of Food Science and Technology, 2021,21(10):197-205.
Niu C Y, Zhang Y Z, Yang J Y, et al. Resrach on the Collaborative Value Assignment of Plasmid Nucleic Acid Refernce Materials Based on Digital PCR［J］. Acta Metrologica Sinica, 2021,42(11):1522-1527.
Wang Y, Yang T M, Cheng B Y, et al. Current Research on Domestic and International Reference Materials of Dairy Products［J］. The Food Industry, 2018,39(9):223-226.
Song H Z, Wei W C, Ma Q, et al. Proficiency Testing Results and Analysis of Salmonella Detection Ability in Milk Powde［J］. China Food Safety Magazine, 2021(18):131-132.
［49］
GB 4789.18—2010 食品安全国家标准 食品微生物学检验 乳与乳制品检验［S］.
Sui Z W, Wang Z Q, Liu S Y, et al. Research Progress and Development Trend of Metrological Techniques for Microbiology［J］. Metrology Science and Technology, 2021,65(6):54-59.
2023, Vol. 44 
