Acta Metrologica Sinica  2023, Vol. 44 Issue (3): 401-409    DOI: 10.3969/j.issn.1000-1158.2023.03.12
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The Research of Quantification for HPV16 and HPV18(E6/E7) RNA Pseudovirus Nucleic Acid Reference Materials
LI Hui-jie1,CHEN Gui-fang3,GAO Yun-hua2,YANG Jia-yi2,DONG Lian-hua2
1. Shenzhen Institute for Technology Innovation, National Institute of Metrology, Shenzhen, Guangdong 518107,China
2. Center for Advanced Measurement Science, National Institute of Metrology, Beijing 100029,China
3. College of Chemistry Engineering, Shenyang University of Chemistry Technology,Shenyang, Liaoning 110142, China
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Abstract  Pseudoviruses containing E6/E7 RNA sequences of HPV16 and HPV18 were selected as reference materials. One-step reverse transcription digital PCR method was used to quantify content of E6/E7 RNA. HPV16 and HPV18 (E6/E7) RNA pseudoviruses were prepared and certificated (NIM-RM5231 and NIM-RM5232), which showed good uniformity and stability. The reference values with expanded uncertainty of the two reference materials were: (9.7±1.8)×102copies/μL, (5.9±1.1)×102copies/μL. The values were verified by multiple laboratories, and the relative standard deviations of the determination results were all less than 10%. The reference materials can provide reference for the quality control of the related detection of high-risk HPV E6/E7 RNA.
Key wordsmetrology      human papilloma virus (HPV)      nucleic acid detection      reference material      one-step reverse transcriptase      digital PCR technology     
Received: 18 July 2022      Published: 08 March 2023
PACS:  TB99  
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LI Hui-jie
CHEN Gui-fang
GAO Yun-hua
YANG Jia-yi
DONG Lian-hua
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LI Hui-jie,CHEN Gui-fang,GAO Yun-hua, et al. The Research of Quantification for HPV16 and HPV18(E6/E7) RNA Pseudovirus Nucleic Acid Reference Materials[J]. Acta Metrologica Sinica, 2023, 44(3): 401-409.
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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2023.03.12     OR     http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2023/V44/I3/401
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