SARS-CoV-2基因组RNA标准物质的研制

doi:10.3969/j.issn.1000-1158.2021.02.20

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Abstract High and low concentrations of reference material of SARS-CoV-2 genomic RNA were developed(GBW(E)091098 and GBW(E)091099)based on the inactivated RNA extraction of the novel coronavirus from infected patients as the potential material,of which the concentrations of E,ORF1ab and N gene were qualified by digital PCR. The reference values with expanded uncertainty of high concentration of reference material were(1.06±0.11)×10³copy/μL,(8.96±0.61)×10²copy/μL and(1.73±0.1)×10³copy/μL,respectively. And those for the low concentration of reference material were(1.29±0.26)×10²copy/μL,(9.5±1.8)×10¹copy/μL and(2.05±0.31)×10²copy/μL,respectively. The reference material here developed had been widely applied to method establishment of nucleic acid detection for SARS-CoV-2,quality control for detection kits and testing laboratory.

Key words： metrology SARS-CoV-2 reference material real-time quantitative PCR digital PCR

Received: 27 July 2020 Published: 18 February 2021

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	Articles by authors
	WANG Di
	WANG Zhi-dong
	WU Xiao
	NIU Chun-yan
	DONG Lian-hua
	DAI Xin-hua
	GAO Yun-hua

Cite this article:

WANG Di,WANG Zhi-dong,WU Xiao, et al. Development of Reference Material of SARS-CoV-2 Genomic RNA[J]. Acta Metrologica Sinica, 2021, 42(2): 259-264.

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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2021.02.20 OR http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2021/V42/I2/259

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