Abstract Static weighing method is commonly used in laboratory to measure the volume of micro-liquid,The accuracy of this method is 2.18% and the repeatability is 1.85% for the volume of 1μL. however, it can not be carried out outside the laboratory. The accuracy and repeatability of the existing optical method for measuring micro-liquid volumetry in 1μL are 6.09% and 1.31%. The mentioned method can realize on-line detection, but its accuracy is not as good as static weighing method. A new method for calibrating the capacity reference standard of this method was found by studying and analyzing the method of optical measurement of micro-liquid capacity. The calibration method combines the static weighing method with the optical measurement method, and takes the static weighing method as a new capacity reference standard instead of the original standard liquid of optical method. The new calibration method extends the reference standard from point to line in principle, and can improve the accuracy of optical measurement at 10μL point to 2.014% and repeatability to 1.30%.
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2020, Vol. 41 
