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| Research on Characterization Method for Library DNA Reference Material for Next Generation Sequencing |
| WANG Xia1,OUYANG Yan-yan1,WANG Jing1,WANG Shang-jun2,DONG Lian-hua1 |
1. National Institute of Metrology, Beijing 100029, China
2. Nanjing Institute of Measurement and Testing Technology, Nanjing, Jiangsu 210049, China |
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Abstract Next generation sequencing (NGS) have been increasingly used in many applications. The accuracy of quantification of DNA library can directly affect the quality of the sequencing data. Thus, it is very important to develop DNA library quantification quality control method. A SYBR Green dye based digital PCR method was established to characterize the DNA library quantification reference material for Illumine NGS platform. The suitability of the proposed reference material was evaluated. The result showed that it is fit for the purpose of usage as standard curve to quantify the DNA library for illumina NGS.The results showed that the five levels of library quantitative reference materials have good linearity, and can be used as the standard curve to quantify the DNA library for Illumina NGS.
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Received: 13 December 2018
Published: 10 October 2020
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2020, Vol. 41 
