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| Equivalent Simulation and Reference Material of the Bacterial Colonies |
| CHEN Ling,HUANG Yan-jie,ZHOU Jin-yan,WU Xiang-lei,HUANG Min-han,XU Jun-bin,LUO Xu-dong |
| Guangdong Provincial Key Laboratory of Modern Geometric and Mechanical Metrology Technology, Guangdong Provincial Institute of Metrology, Guangzhou,Guangdong 510405, China |
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Abstract The bacterial colony itself is unstable and inconvenient to carry, so it is not suitable for use as a reference material for calibration of colony counters. Colony simulation images were used to represent colonies. According to the characteristics of colonies, colony images of different sizes and uneven distribution were processed into pictures, and the total number and size of simulated colonies were simulated to simulate standard colony materials. The total number and size of the simulated bacterial colony were equivalent to the simulated bacterial colony reference materials.A stratified random sampling method was used to study the homogeneity and stability of the reference materials.The results showed that the prepared reference materials had good homogeneity and stability.Through rigorous calculation and data processing, the total number and size of the simulated bacterial colony were determined and the uncertainty was calculated.The series of reference materials could be stored and carried easily, so they were suitable for bacterial colony counter calibration and analysis method evaluation, and have been certified as the secondary national certified reference materials with the number of GBW(E) 130586~GBW(E) 130590.
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Received: 17 May 2019
Published: 19 December 2019
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2020, Vol. 41 
