荧光薄膜材料荧光特性对荧光显微镜分析测量效果的影响

doi:10.3969/j.issn.1000-1158.2019.04.32

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Abstract The accuracy of the data measured by the fluorescence microscope is influenced by the linear dynamic range, the uniformity of the light field, and the stability of the light source. Fluorescence microscopy experiments were carried out on fluorescent microscopy materials, and it was found that fluorescence characteristics such as fluorescence linearity, uniformity and stability are important for fluorescence microscopy analysis. The spectral characteristics, linearity, uniformity, and light stability of fluorescent film materials were studied, a method for measuring the fluorescent properties of fluorescent film materials was established, a preliminary exploration of the calibration method for fluorescent microscopes was made, and the foundation for the calibration of fluorescent microscopes was laid.

Key words： metrology thin filmmaterials fluorescence measurement fluorescent properties fluorescence microscopy calibration

Received: 07 February 2018 Published: 10 June 2019

PACS:

TB99

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	Articles by authors
	XU Pei-pei
	ZHANG Ling
	HUANG Fei
	WANG Fei
	YE Zi-hong

Cite this article:

XU Pei-pei,ZHANG Ling,HUANG Fei, et al. Effect of Fluorescence Properties of Fluorescent Film Materials on the Measurement Results of Fluorescence Microscope Analysis[J]. Acta Metrologica Sinica, 2019, 40(4): 742-748.

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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2019.04.32 OR http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2019/V40/I4/742

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