克伦特罗残留检测免疫芯片的研制

doi:10.3969/j.issn.1000-1158.2015.06.23

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Abstract Immunochip is designed in order to realize the massive detection of clenbuterol residue.Slide glass surface as the carrier is modified by APTES-DGA.The clenbuterol antibody is coupled to the slide glass surface.Glass surface active groups are blocked by bovine serum albumin. The samples and standards, horseradish peroxidase labeled clenbuterol are added into the immunochip.CLB and CLB-HRP are combined competitively with Ab (CLB).Chemiluminescence reaction is catalyzed by the horseradish peroxidase and optical signal value is detected Sampling volume, incubation temperature, incubation time, concentration of washing solution, chemiluminescence integral time are tested.The results show that glass slide with APTESDGA has a better signal uniformity and antibody coupling ability. In the detection process, immunochip signal is stable when sampling volume is 50 μL, incubation temperature is 37 ℃, incubation time is 30 min, wash solution concentration is 10 fold diluent and chemiluminescence reader integral time is 60 s. The method is fast, simple and accurate by immunochip analysis for clenbuterol residue.

Key words： metrology immunochip drug residue detection； clenbuterol chemiluminescence

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TB99

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	Articles by authors
	SUN Lai-yu
	QIAN Kun
	SHAO Chao-gang
	YIN Li
	YAN Qiong-qiong
	LU Yun-hua
	YANG Jin-tian

Cite this article:

SUN Lai-yu,QIAN Kun,SHAO Chao-gang, et al. Development of Immunochip for Detection of Clenbuterol Residue[J]. Acta Metrologica Sinica, 2015, 36(6): 657-661.

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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2015.06.23 OR http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2015/V36/I6/657

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