|
|
|
| Extract Bone Trabecular of Hematoxylin-eosin Staining Dyeing Pathological Section by Visual Model |
| YE Han-xiao1, JIN Hong-ting1, YUAN Xin2, JIANG Yi-fa1 |
1.Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China;
2.Zhjiang University, Hangzhou, Zhejiang 310013, China |
|
|
|
|
Abstract To extract trabecular bone of hematoxylin-eosin staining staining bone tissue pathological slices,for quantitative detection of trabecular bone area,create a polar diffusion model,to let computer extract any hematoxylin-eosin staining dyeing bone slice trabecular bone,divide the boundary of the trabecular bone with bone cells and bone cortex.Extract a pixel’s RGB values of background,use RGB color value of all pixels with the background pixels to calculate 3d euclidean distance,get threshold,to achieve image binarization segmentation,by area filling get trabecular bone.Results show that the visual model can get the boundary of the region of interest well.Measurement accuracy and speed are significantly improved.Results of measurement relative to the conventional software the robustness improve over 95%,reduce human-computer interaction,to reduce the blindness of testing.
|
|
|
|
|
|
［1］ 李建赤,黄必留,徐自强.骨组织形态计量学在临床骨病应用中的研究与进展［J］.中国组织工程研究与临床康复,2011,（3）:6607-6610.
［2］ 付鑫,马剑雄,董宝康,马信龙.骨微结构检测方法研究进展［J］.中国骨与关节外科,2009,(6):509-514.
［3］ 曹立,雍宜民.老年股骨颈骨折患者骨密度、骨形态计量学的研究［J］.中华创伤杂志,2000,(8):488-491.
［4］ 邱明才,杨宝恩,王觉英,等.正常国人髂骨形态计量参数的研究［J］.中华医学杂志,1990,(12):685.
［5］ 郭昭庆,党耕町,王志国,等.正常人髂嵴活检标本的骨组织形态计量学分析［J］.中华骨科杂志,1995,(4):228-230.
［6］ 邱明才,戴晨琳,李玉坤,等.代谢性骨病学［M］.北京:人民卫生出版社,2012:436-438.
［7］ Quest D,Gayer C,Hering P.Depth measurements of drilled holes in bone by laser triangulation for the field of oral implantology［J］. Journal of Applied Physics,2012,111(1): 013106.
［8］ Gundersen H J G, et al. Distribution of membrane thickness determined by lineal analysis［J］.Journal of Microscopy,1978,113(1):27-43.
［9］ OsteoMeasure TM software［OL］. http://www.osteometrics.com/product_info.htm,2012-09-20.
［10］ Bioquant OsteoⅡ software［OL］. http://osteo.bioquant.com/home,2012-08-02.
［11］ Droke E, Hager K, Lerner M, et al. Soy isoflavones avert chronic inflammation-induced bone loss and vascular disease［J］. Journal of Inflammation,2007, (4):17.
［12］ 吴凤和.基于计算机视觉测量技术的图像轮廓提取方法研究［J］.计量学报,2007,28(1):18-22. |
|
|
|
2014, Vol. 35 
