Research on the Metrology Traceability Technology of Surface Plasmon Resonance Biomolecular Interaction Analysis

LI Lanying; TAO Qing; LIU Gang

doi:10.3969/j.issn.1000-1158.2025.05.18

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Acta Metrologica Sinica ›› 2025, Vol. 46 ›› Issue (5) : 748-753. DOI: 10.3969/j.issn.1000-1158.2025.05.18

Research on the Metrology Traceability Technology of Surface Plasmon Resonance Biomolecular Interaction Analysis

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Abstract

According to the core principle of surface plasma resonance （SPR） biomolecular interaction analysis， a metrological traceability method was proposed， which takes the refractive index as the theoretical basis and combines sucrose reference materials. Firstly， sucrose standard solutions were prepared and quantified using a refractometer. Then， the refractive index values were traceable to the Abbe refractometer standard block， thereby establishing the calibration basis for the refractive index， realizing repeatability detection of the refractive index， linear calibration within the range of refractive index standard values from 1.330 to 1.400， and analysis of the analyzer’s relative baseline noise and relative baseline drift. Finally， using the purity reference materials of sucrose with gradient dilution， the calibration of the detection limit was completed. For the first time， a comprehensive， scientific， and effective metrological traceability method for SPR biomolecular interaction analysis has been established， which can be applied to the metrological calibration of related instruments in the fields of basic life science research and biopharmaceutical development.

Key words

biometrology / biomolecular interaction / surface plasmon resonance / refractive index / sucrose reference materials / calibration / uncertainty

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LI Lanying , TAO Qing , LIU Gang. Research on the Metrology Traceability Technology of Surface Plasmon Resonance Biomolecular Interaction Analysis[J]. Acta Metrologica Sinica. 2025, 46(5): 748-753 https://doi.org/10.3969/j.issn.1000-1158.2025.05.18

References

List( Publishing order | Descend order by publishing year | Descend order by cited within ) Chart analysis

1	LI C， ZHAN W， YANG Z， et al. Broad neutralization of SARS-CoV-2 variants by an inhalable bispecific single-domain antibody ［J］. Cell， 2022， 185 （8）： 1389-1401 e1318. Cited in this article [1]

2	BAIMANOV D， WANG J， ZHANG J， et al. In situ analysis of nanoparticle soft corona and dynamic evolution ［J］. Nature Communications， 2022， 13 （1）： 5389-5403.

3	XU X W， ZHU Y， SONG J Z， et al. Selective photoaffinity probe for monitoring marnesoid X receptor expression in cultured cells ［J］. Analytical Chemistry， 2022， 94 （30）： 10722-10729.

4	POUDYAL M， PATEL K， GADHE L， et al. Intermolecular interactions underlie protein/peptide phase separation irrespective of sequence and structure at crowded milieu ［J］. Nature Communications， 2023， 14 （1）： 6199-6220.

5	QIN W， CHO K F， CAVANAGH P E， et al. Deciphering molecular interactions by proximity labeling ［J］. Nature Methods， 2021， 18 （2）： 133-143. Cited in this article [1]

6	ZHOU J， QI Q， WANG C， et al. Surface plasmon resonance （SPR） biosensors for food allergen detection in food matrices ［J］. Biosensors and Bioelectronics， 2019， 142： 111449-111464. Cited in this article [1]

7	SINGH Y， PASWAN M K， RAGHUWANSHI S K. Sensitivity enhancement of SPR aensor with the black phosphorus and graphene with Bi-layer of gold for chemical sensing ［J］. Plasmonics， 2021， 16 （5）： 1781-1790.

8	HOSSAIN M B， ISLAM M M， ABODULRAZAK L F， et al. Graphene-coated optical fiber SPR biosensor for BRCA1 and BRCA2 breast cancer biomarker detection： a numerical design-based analysis ［J］. Photonic Sensors， 2020， 10 （1）： 67-79.

9	GUO Z Q， ZHENG T， CHEN B， et al. Small-molecule targeting of E3 ligase adaptor SPOP in kidney cancer ［J］. Cancer Cell， 2016， 30 （3）： 474-484.

10	应必仕，朱君，任益弘，等. 几种表面等离子体共振传感技术的研究进展［J］. 激光杂志， 2021， 42 （9）： 1-5. YING B S， ZHU J， REN Y H， et al. Research progress of surface plasmon resonance sensors ［J］. Laser Journal， 2021， 42（9）： 1-5. Cited in this article [1]

11	国家药典委员会. 中华人民共和国药典2020年版（三部）［M］.北京：中国医药科技出版社， 2020. Cited in this article [1]

12	The United States Pharmacopeia Convention. General information/（1105） Immunological test methods -surface plasmon resonance ［S］. 2023.

13	The Japanese Pharmacopoeia. General information/ biotechnological/Biological products-surface plasmon resonance ［S］. 2016. Cited in this article [1]

14	Food and Drug Administration. Assay Development and Validation for Immunogenicity Testing of Therapeutic Protein Products ［M］.New York：ImprintJenny Stanford Publishing， 2018. Cited in this article [1]

15	CHEN L， LV D， CHEN X， et al. Biosensor-based active ingredients recognition system for screening STAT3 ligands from medical herbs ［J］. Analytical Chemistry， 2018， 90 （15）： 8936-8945. Cited in this article [1]

16	LIAO L X， SONG X M， WANG L C， et al. Highly selective inhibition of IMPDH2 provides the basis of antineuroinflammation therapy ［J］. Proceedings of the National Academy of Sciences of the United States of America， 2017， 114 （29）： E5986-E5994.

17	PAN Z， CHEN J， XIAO X， et al. Characterization of a novel bispecific antibody targeting tissue factor-positive tumors with T cell engagement ［J］. Acta Pharmaceutica Sinica B， 2022， 12 （4）： 1928-1942.

18	MATSUNAGA R， UJIIE K， INAGAKI M， et al. High-throughput analysis system of interaction kinetics for data-driven antibody design ［J］. Scientific Reports， 2023， 13 （1）： 19417-19426.

19	车洁，邵祝军. 表面等离子体共振技术在疫苗领域中的应用［J］. 中国疫苗和免疫， 2022， 28 （4）： 475-480. CHE J， SHAO Z J. Application of the surface plasmon resonance technique in the vaccine field ［J］. Chinese journal of vaccines and immunization， 2022， 28 （4）： 475-480. Cited in this article [1]

20	SU P， HE Z， WU L， et al. SI-traceable calibration-free analysis for the active concentration of G2-EPSPS protein using surface plasmon resonance［J］. Talanta， 2017， 178（2018）：78-84. Cited in this article [1]

21	LI K， AN N， WU L， et al. Absolute quantification of microRNAs based on mass transport limitation under a laminar flow SPR system［J］. Biosens Bioelectron， 2024， 244（15）：115776-115786. Cited in this article [1]

22	罗世闻，武利庆，杨彬，等. 表面等离子体共振技术及其在蛋白质活性浓度绝对定量中的应用［J］. 计量学报， 2023， 44 （11）： 1770-1775. LUO S W， WU L Q， YANG B， et al. Surface plasmon resonance and its application in absolute quantification of active protein concentration［J］. Acta Metrologica Sinica， 2023， 44 （11）： 1770-1775. Cited in this article [1]