基于原子磁力计的免疫层析试纸定量测量方法

doi:10.3969/j.issn.1000-1158.2025.02.17

Abstract
Figure/Table
References (19)
Related Citation (9)

Download: PDF (0 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Lateral flow immunoassay （LFIA） is widely used in rapid disease diagnosis due to its advantages such as easy operation，small sample dosage，and easy interpretation of results.However，how to quantitatively measure the target is still a key problem in LFIA application of.A quantitative measurement method of LFIA paper based on an atomic magnetometer was proposed using superparamagnetic nanoparticleses （MNP） as LFIA markers combined with quantum Magnetic sensing technology.The residual magnetic flux density spectrum was obtained by linear scanning of the paper to be tested by the atomic magnetometer.The logarithmic linear relationship between the magnetic moment and the concentration of the object was analyzed，and the amount of MNP contained was calculated to achieve quantitative measurement.When the distance between the magnetometer and the sample to be measured was 9mm，the linear range of Pro-Gastrin Releasing Peptide （Pro-GRP） LFIA test paper was 10²~10⁴ pg/mL with the lowest detection limit of 10² pg/mL.The number of MNP at this concentration is estimated to be 1.057×10⁴.

Key words： biometrology lateral flow immunoassay atomic magnetometer superparamagnetic nanoparticles quantitative measurement

Received: 19 February 2024 Published: 04 March 2025

PACS:	TB99
	TB972

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors

Cite this article:

URL:

http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2025.02.17 OR http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2025/V46/I2/280

16	BUDKER D， ROMALIS M. Optical magnetometry ［J］. Nature Physics， 2007， 3（4）： 227-234.
2	SHENG W， GUO J， LIU C， et al. Quantitative determination of four mycotoxins in cereal by fluorescent microsphere based immunochromatographic assay ［J］. Journal of the Science of Food and Agriculture， 2023， 103（8）： 4017-4024.
4	KIM D， WANG S X. A magneto-nanosensor immunoassay for sensitive detection of Aspergillus fumigatus allergen Asp f 1 ［J］. IEEE Transactions on Magnetics， 2012， 48（11）： 3266-3268.
3	王策，刘晓婷，王成彬. 基于超顺磁性磁珠的免疫层析技术的应用［J］. 临床检验杂志（电子版）， 2015， 4（2）： 859-866. WANG C， LIU X， WANG C. Application of immunochr-omatographic techniques based on superparamagnetic magnetic beads ［J］. Chinese Journal of Clinical Laboratory Science（Electronic version）， 2015， 4（2）： 859-866.
5	NGUYEN V T， SONG S， PARK S， et al. Recent advances in high-sensitivity detection methods for paper-based lateral-flow assay ［J］. Biosensors and Bioelectronics， 2020， 152： 112015.
6	BARNETT J M， WRAITH P， KIELY J， et al. An inexpensive， fast and sensitive quantitative lateral flow magneto-immunoassay for total prostate specific antigen ［J］. Biosensors， 2014， 4（3）： 204-220.
8	MARQUINA C， DE T J M， SERRATE D， et al. GMR sensors and magnetic nanoparticles for immunochroma-tographic assays ［J］. Journal of Magnetism and Magnetic Materials， 2012， 324（21）： 3495-3498.
10	WANG K， LI T， CAO B， et al. Simulation and improvements of a magnetic flux sensor for application in immunomagnetic biosensing platforms ［J］. Sensors and Actuators A： Physical， 2022， 333： 113299.
12	UCHIDA S， HIGUCHI Y， UEOKA Y， et al. Highly Sensitive Liquid-Phase Detection of Biological Targets With Magnetic Markers and High Tc SQUID ［J］. IEEE Transactions on Applied Superconductivity， 2014， 24（4）： 1-5.
13	WIEKHORST F， STEINHOFF U， EBERBECK D， et al. Magnetorelaxometry assisting biomedical applications of magnetic nanoparticles ［J］. Pharmaceutical Research， 2012， 29： 1189-1202.
15	ENPUKU K， TAMAI Y， MITAKE T， et al. AC susceptibility measurement of magnetic markers in suspension for liquid phase immunoassay ［J］. Journal of Applied Physics， 2010， 108（3）： 371.
18	WANG B， PENG T， JIANG Z， et al. Highly Sensitive and Quantitative Magnetic Nanoparticle-Based Lateral Flow Immunoassay with an Atomic Magnetometer ［J］. ACS Sensors， 2023， 8（12）： 4512-4520.
1	陶晴，王乐乐，彭涛，等. 量子点掺杂荧光凝胶标准试纸条研制及荧光免疫分析仪校准应用［J］. 计量学报， 2023， 44（12）： 1919-1924. TAO Q， WANG L， PENG T， et al. Development of Quantum Dot-Doped Fluorescent Gel Reference Strip and Its Application in Calibration of Fluorescence Immunoassay Analyzer ［J］. Acta Metrologica Sinica， 2023， 44（12）： 1919-1924.
9	SHEN W， SCHRAG B D， CARTER M J， et al. Quantitative detection of DNA labeled with magnetic nanoparticles using arrays of MgO-based magnetic tunnel junction sensors ［J］. Applied Physics Letters， 2008， 93（3）： 3294.
11	WORKMAN S， WELLS S K， PAU C P， et al. Rapid detection of HIV-1 p24 antigen using magnetic immuno-chromatography （MICT）［J］. Journal of Virological Methods， 2009， 160（1-2）： 14-21.
19	LI J， QUAN W， ZHOU B， et al. SERF atomic magneto-meterrecent advances and applications： A review ［J］. IEEE Sensors Journal， 2018， 18（20）： 8198-8207.
7	CAO B， WANG K， XU H， et al. Development of magnetic sensor technologies for point-of-care testing： Fundamentals， methodologies and applications ［J］. Sensors and Actuators A： Physical， 2020， 312： 112130.
14	KUMA H， OYAMADA H， TSUKAMOTO A， et al. Liquid phase immunoassays utilizing magnetic markers and SQUID magnetometer ［J］. Clinical Chemistry and Laboratory Medicine， 2010， 48（9）： 1263-1269.
17	YAO L， JAMISON A C， XU S. Scanning imaging of magnetic nanoparticles for quantitative molecular imaging ［J］. Angewandte Chemie International Edition， 2010， 49（41）： 7493-7496.