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| Establishment of Quantitative PCR Methods for the Detection of Genetically Modified Maize MZIR098 |
| WEN Hong-tao1,YANG Yang1,DING Yi-jia1,GUAN Hai-tao1,YUAN Ran1,ZHANG Rui-ying1,LI Ling-yan3,LIANG Jin-Gang2,WANG Jing1 |
1. Quality & Safety Institute of Agricultural Products, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang 150086, China
2. Development Center of Science and Technology, Ministry of Agriculture and Rural Affairs of the People’ Republic of China, Beijing 100176, China
3. Beijing Vocational College of Agriculture, Beijing 102442, China |
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Abstract Primers and probes were designed based on the 5′flanking sequence information of transgenic insect resistant and herbicide tolerant maize MZIR098. Subsequently, a double quantitative assay for the transformant specificity of maize MZIR098 was established through qPCR and 3D-dPCR platforms. After testing, it was found that the repeatability (relative standard deviation) of the two detection methods, qPCR and 3D-dPCR, was less than 25%, which met the relevant standards of transgenic detection methods, and there was no significant difference between the detection limit and the quantification limit. The above results showed that the two methods can meet the basic needs of quantitative detection of transgenic plants, and can provide a new reference method for accurate and efficient detection of maize MZIR098 and its products in the future.
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Received: 01 August 2022
Published: 08 March 2023
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2023, Vol. 44 
