Cell Growth and Drug Effect Differences Induced by Inoculum Number Variations and Comparison of Cell Counting Methods

XUE Zhi-chao; ZENG Jia-ming; LI Yong-shu; ZHAO Jia-wei; ZHAO Yang; DAI Xin-hua; GONG Xiao-yun

doi:10.3969/j.issn.1000-1158.2023.03.04

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Acta Metrologica Sinica ›› 2023, Vol. 44 ›› Issue (3) : 341-349. DOI: 10.3969/j.issn.1000-1158.2023.03.04

Cell Growth and Drug Effect Differences Induced by Inoculum Number Variations and Comparison of Cell Counting Methods

XUE Zhi-chao¹,ZENG Jia-ming¹,LI Yong-shu²,ZHAO Jia-wei¹,ZHAO Yang¹,DAI Xin-hua¹,GONG Xiao-yun¹

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Abstract

Hela cells were used to investigate the changes in cell growth rate and the influence of drug effect induced by the cell number differences at the cell seeding step. The experimental results indicated that the larger the initial number of cells, the higher the density after adhesion, the faster the growth rate, and the lower the inhibitory effect of drugs on its growth. Therefore, the accuracy and efficiency of cell counting are of great importance for in vitro studies. Three commonly used cell counting methods were compared including the cell counting plate method, the cell counting apparatus method, and the flow cytometry method. Their linear regression R2 were 0.9835, 0.9997, and 0.9960 respectively. Under the same condition, the cell counting apparatus method held the highest accuracy. In addition, indirect methods such as crystal violet staining, basal area photography, protein content estimation, and protein reference GAPDH measurement were also evaluated in to provide data reference for cell number measuring in biological experiments.

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metrology / cell metrology / cell number / growth rate / cell counting plate method / cell counting apparatus method / flow cytometry method

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XUE Zhi-chao,ZENG Jia-ming,LI Yong-shu,ZHAO Jia-wei,ZHAO Yang,DAI Xin-hua,GONG Xiao-yun. Cell Growth and Drug Effect Differences Induced by Inoculum Number Variations and Comparison of Cell Counting Methods[J]. Acta Metrologica Sinica. 2023, 44(3): 341-349 https://doi.org/10.3969/j.issn.1000-1158.2023.03.04

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