生物气溶胶监测仪的校准方法比较

doi:10.3969/j.issn.1000-1158.2022.01.22

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Abstract The bioaerosol monitor detect biological substances in the air based on laser-induced fluorescence, which is of great significance for disease prevention and early warning of biological terrorism. The study found that the current domestic bioaerosol monitor cant distinguish different bacterial states when collecting biological particles in the air. The bioaerosol monitor detect the total number of bacteria. Through real-time quantitative PCR and colony counting method to detect the microorganisms in the BioSampler, it was found that the total number of bacteria and the number of cultivable bacteria were significantly different. Observation by scanning electron microscope and fluorescence microscope found that the impact and centrifugation of the sampler affected the viability of the microorganisms, so it is not suitable to calibrate the bioaerosol monitor by the colony culture method.

Key words： metrology bioaerosol detector colony counting method real-time quantitative PCR fluorescence microscope scanning electron microscope

Received: 11 March 2021 Published: 06 January 2022

PACS:

TB99

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	Articles by authors
	TIAN Ying
	ZHANG Guo-cheng
	WU Dan
	LIU Jia-qi
	LI Jing-jing
	HUO Sheng-wei
	SHEN Shang-yi
	PAN Yi-ting

Cite this article:

TIAN Ying,ZHANG Guo-cheng,WU Dan, et al. Comparison of Biological Calibration Methods of Bioaerosol Monitor[J]. Acta Metrologica Sinica, 2022, 43(1): 140-144.

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http://jlxb.china-csm.org:81/Jwk_jlxb/EN/10.3969/j.issn.1000-1158.2022.01.22 OR http://jlxb.china-csm.org:81/Jwk_jlxb/EN/Y2022/V43/I1/140

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