1.National Institute of Metrology, Beijing 100013, China
2.Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, China
3.Beijing University of Chemical Technology, Beijing 100029, China
Abstract：Three levels of genetically modified soybeans Mon89788 matrix reference material are developed. Genetically modified soybean genomic DNA extraction and quantitative PCR of transgenic components methods are researched. A multi-laboratory verification and uncertainty evaluation are carried out too.The analysis of multi-laboratory value verification shows that in three different levels of genetically modified soybean Mon89788 reference materials, the transgenic gene / endogenous genes reference value and uncertainty is 0.35%±0.17%,0.61%±0.14%,3.17%±1.70% (k=2), respectively.This set of reference materials that the matrix is soybean grand can be used for the quantitative detection of genetically modified soybeans content.
［1］ISO Guide 31-2000,Reference materials-contents of certificates and labels［S］.
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2011, Vol. 32 
